Organic/inorganic double-layered shells for multiple cytoprotection of individual living cells

다국어 초록 (Multilingual Abstract)

<▼1><P><I>S. cerevisiae</I> encapsulated with a poly(norepinephrine)/silica double-layered shell showed multiple resistance to enzymatic attack, desiccation, and UV-C irradiation. The biochemical response of the encapsulated yeast may also contribute to the UV-C resistance.</P></▼1><▼2><P>The cytoprotection of individual living cells under <I>in vitro</I> and daily-life conditions is a prerequisite for various cell-based applications including cell therapy, cell-based sensors, regenerative medicine, and even the food industry. In this work, we use a cytocompatible two-step process to encapsulate <I>Saccharomyces cerevisiae</I> in a highly uniform nanometric (<100 nm) shell composed of organic poly(norepinephrine) and inorganic silica layers. The resulting cell-in-shell structure acquires multiple resistance against lytic enzyme, desiccation, and UV-C irradiation. In addition to the UV-C filtering effect of the double-layered shell, the biochemical responses of the encapsulated yeast are suggested to contribute to the observed UV-C tolerance. This work offers a chemical tool for cytoprotecting individual living cells under multiple stresses and also for studying biochemical behavior at the cellular level.</P></▼2>