Substitution effect of sea tangle (Saccharina japonica) with beet leaf by-product in feed on the growth and chemical composition of the soft body of juvenile abalone (Haliotis discus hannai Ino 1952) subjected to air exposure = 전복용 배합사료내 비트잎부산물의 다시마(Saccharina japonica)대체가 참전복 (Haliotis discus hannai) 치패의 성장과 체조성 및 공기 노출시 스트레스 내성에 미치는 영향

다국어 초록 (Multilingual Abstract)

Dietary substitution effect of beet leaf by-product (BLB) for sea tangle (Saccharina japonica) on the growth and soft body composition of abalone (Haliotis discus hannai) subjected to air exposure was determined. A total of 1260 juvenile abalone was randomly distributed into 18 containers. Five experimental diets were prepared. The control (Con) diet contained 20% S. japonica. Twenty five, 50, 75 and 100% S. japonica were substituted with equal amount of BLB, referred to as the BLB25, BLB50, BLB75 and BLB100 diets, respectively. The dry S. japonica was also prepared to compare effect of formulated diets on growth of abalone. The experimental diets were fed to triplicate groups of abalone for 16 weeks, and then exposed to air exposure for 24 h. Survival of abalone was monitored for the next 6 days post observation period after the 24-h air exposure. Weight gain and specific growth rate (SGR) of abalone fed with formulated diets were greater than those of abalone fed with the S. japonica. The greatest weight gain and SGR were obtained in abalone fed the BLB25 diet, followed by the BLB50, BLB75, BLB100 and Con diets, in that order. The maximum values of weight gain, SGR, shell length and shell width of abalone were estimated to be approximately 42.3, 43.5, 39.8 and 33.7% of substitution level of BLB for S. japonica in abalone feed. The chemical composition of abalone soft body was not affected by the experimental diets. Survival of abalone fed with formulated diets was higher than that of abalone fed with the S. japonica at the end of the 6-day post observation period. In conclusion, S. japonica could be completely replaced with BLB in abalone feed without retardation of growth and survival of abalone subjected to the 24-h air exposure. The greatest weight gain and SGR were obtained in abalone fed the BLB25 diet.

목차 (Table of Contents)

• List of Tables ⅲ
• List of Figures ⅳ
• Abstract (in English) ⅵ
• 1. Introduction 1
• 2. Materials and Methods 5
• 2.1 Preparation of the BLB 5
• 2.2 Preparation of the experimental diets 5
• 2.3 Preparation of abalone and rearing condition 7
• 2.4 Sample collection and measurement of abalone growth 8
• 2.5 Chemical analysis of the experimental diets and soft body of abalone 8
• 2.6 Water stability of the experimental diets 9
• 2.7 Observation of survival of abalone subjected to air exposure stressor 9
• 2.8 Statistical analysis 9
• 3. Results 11
• 3.1 Water stability of the experimental diets over time 11
• 3.2 Performance of abalone 16
• 3.3 Biological parameter of abalone 19
• 3.4 Chemical composition of soft body of abalone 21
• 3.5 Survival of abalone subjected to 24-h air exposure stressor 21
• 4. Discussion 24
• 5. Conclusion 28
• References 29
• Abstract (in Korea) 43