아가리쿠스 버섯 균사체 추출물 및 분획물의 항돌연변이원성 및 세포 독성 효과 = The Antimutagenic Effects and Cytotoxic Activities of Agaricus blazei Murill Mycelium Extracts and Fractions

다국어 초록 (Multilingual Abstract)

This study was performed to observe the antioxidative effects, antimutagenic capacity, and cytotoxic activity of the 70% ethanol extract, and fractions, of Agaricus blazei Murill mycelium, using DPPH free radical scavenging ability, the Ames test, and SRB assay, respectively. Among the fractions, ethyl acetate showed the most effective antioxidative capacity according to the $RC_{50}$(73.6 $\mu$g/mL) of the scavenging effect on the DPPH radical. The inhibition rate of both the aqueous fraction and 70% ethanol extract(200 $\mu$g/plate) toward the Salmonella typhimurium TA100 strain was 94.6%, and it was 89.4% against the mutagenesis induced by MNNG(0.4 $\mu$g/plate). In addition, an identical concentration of the 70% ethanol extract in the TA98 strain, and the ethyl acetate fraction in the TA100 strain, showed inhibition rates of 80.3% and 76.9%, respectively, the highest activities against the mutagenesis induced by 4NQO(0.15 $\mu$g/plate). The cytotoxic effects of the 70% ethanol extract and its fractions increased with increasing sample concentration against human cervical adenocarcinoma(HeLa), human hepatocellular carcinoma(Hep3B), human breast adenocarcinoma(MCF-7), human stomach adenocarcinoma(AGS), and human lung carcinoma (A549). A 1 mg/mL concentration of the ethyl acetate fraction showed cytotoxicities of 77%, 83.8%, 82.1%, 83.1%, and 92.6% against HeLa, Hep3B, MCF-7, AGS and A549, respectively.

참고문헌 (Reference)

1 "Tumoricidal activity of high molecular weight polysaccharides derived from Agaricus blazie via oral administration in the mouse tumor model" 45 : 246-252, 1998

2 "The effect of mushroom extracts on carbon tetrachloride induced hepatotoxicity in rats" 27 : 326-332, 1998

3 "Studies on biological polysaccharides isolated from Agaricus bisporus (in Korean)" 41 : 60-66, 1998

4 "Structural characterization of a water-soluble β-D-glucan from fruiting bodies of Agaricus blazei Murill" 337 : 1417-1421, 2002

5 "Screening for antioxidant activity of plants and marune algae and its active principles from Prunus daviana. Kor J Pharmacol 24" 299-303, 1993

6 "Polysaccharides in pharmacy. In" 493-497, 1989

7 "Polysaccharides from Agaricus blasei stimulate lymphosyte T-cell subsets in mice" 62 : 434-437, 1998

8 "Physiological activities of Phellinus ribis extracts" 35 : 690-695, 2003

9 "Oxford. pp 130-161." 1988

10 "Mutagenicity of carcinogenic azo dyes and their derivatives. Cancer Letter 1" sei (sei): 91-97, 1975

11 "Mushroom" Kyohaksa, Seoul 3-10, 1998

12 "Isolation and properties of a lectin from the fruiting bodies of Agaricus blazei. Carbohydr Res 183" 150-154, 1988

13 "Inhibitory action of a -β-D-glucan-protein complex isolated from Agaricus blazei Murill on meth a fibrosarcoma-bearing mice and its antitumor mechanism. Jpn J Pharmacol 66" 265-271, 1994

14 "Fractionation and antitumor activity of the water-in-soluble residue of Agaricus blazei fruiting bodies. Carbohyd Res 186" 267-273, 1989

15 "Fraction and antitumor activity of the water insoluble residue of Agaricus blazei fruiting bodies. Carbohydr Res 186" 267-273, 1989

16 "Extraction and purification of bioactive materials from Agaricus blazei fruitiong bodies" 15 : 293-298, 2000

17 "Effects of antioxidative stress antimutagenicity and cytotoxicity of cancer cells in fractional extracts from Ganoderma lucidum Krast" 21 : 759-768, 2005

18 "Effect of hot water extract from Agaricus blasei Murill on antibldy- producing cells in mice" 2 : 1205-1211, 2002

19 "Comparision of 5 microplate colorimetric assay for in vitro cytotoxicity testing and cell proliferation assays" 11 : 49-54, 1997

20 "Chemical compositions of Agaricus blazei Murill fruiting bodies cultivated in a Korean local farm" 13 : 94-98, 1998

21 "Cancer chemoprevention by food" 30 : 59-63, 1997

22 "Antiveoplastic effect of extracts from traditional medicinal plants and various plants. Kor J Pharmacology 25" 171-177, 1994

23 "Antitumor components of Cryptoporus volvatus. Kor J Mycol 10" 111-117, 1982

24 "Antitumor and immunomodulating activities of a β-glucan obtained from liquid cultured Grifola frondosa. Chem Pharm Bull 37" 410-413, 1989

25 "Antitumor activity and some properties of water-insoluble hetero-glycans from the fruiting body of Agaricus blazei Murill. Agric Biol Chem 54" 2897-2905, 1990b

26 "Antitumor activity and some properties of water soluble polysaccharides from fruiting body of Agaricus blazei Murill. Agric Biol Chem 54" 2889-2896, 1990a

27 "Antimutagenic effects of the mushroom Agaricus blazei Murill extracts on V79 cells" 496 : 5-13, 2001

28 "Antimutagenic and cytotoxicity effects of Agaricus blazei Murill extracts" 32 : 1371-1378, 2000

29 "Antimutagenic and bactericidal substances in the fruit body of a Basidiomycete Agaricus blazei. Yakugaku Zasshi 114" 342-350, 1994

30 "Anticomplementary and antitumor activities of the alkal extract from the mycelia of Lentinus edodes IY-105" 18 : 571-579, 1996

31 "Anticom plementary activity of antitumor polysaccharides. Nature New Biol 238" 59-60, 1972

32 "Anticancer activities of the extract from the mycelia of Coriolus versicolor. Kor J Appl Mycrobiol Biotechnol 20" 311-315, 1992

33 "A mushroom extract as an interferon inducer. Ann NY Acad Sci 173" 719-725, 1969