홍화자 분획물이 사염화탄소 유발 간손상 흰쥐에서 지질과산화와 oxygen free radical 제거 효소 활성도에 미치는 영향 = Effect of Subfractions of Carthamus tinctorius L. Semen on the Lipid Peroxidation and Oxygen Free Radical Scavenging Enzyme Activities in CCI₄-induced Hepatotoxic Rats

국문 초록 (Abstract)

저자들은 사염화탄소로 유발된 간손상에 대한 홍화자 메탄올 추출물과 이를 계통 분획한 분획물의 보호 효과를 이미 보고하였다. 본 실험에서는 홍화자 분획물의 간보호 작용에 대한 기전을 살펴보기 위하여 지질과산화와 oxygen free radical 제거 효소 활성을 측정하였고 조직병리학적 분석을 통하여 간보호 효과를 확인하였다. 홍화자 BS-5 분획물은 CCl_4로 간손상을 유발시킨 흰쥐에서 superoxide dismutase의 활성도를 유의적으로 증가시켰으며, in vitro에서의 1, 1-diphenyl -2-picrylhydrazyl radical를 직접적으로 제거하였다. 또한 조직병리학적 분석에서도 홍화자 BS-5분획투여군은 CCl_4 투여로 인한 중심정맥 주변의 ballooning degeneration, 염증 세포 침윤 및 괴사를 완화시켜 간손상 보호 작용을 나타내었으며, 이는 free radical scavenging 작용에 의한 것으로 생각된다.

다국어 초록 (Multilingual Abstract)

Previous studies have shown that methanol extract and its butanol fraction of Carthamus tinctorius L. Semen have the hepatoprotective effect on the CCl_4-induced hepatotoxicity. The hepatoprotective effect fo the subfractions of butanol fraction has been evaluated by analyzing oxygen free radical scavenging enzyme activities and histopathological examinations. In BS-5 subfraction treated group, the activity of superoxide dismutase has been significantly increased as compared with that of CCl_4 treated rats. Antioxidant activity has been evaluated by the examination of the scavenging ativity against 1,1-diphenyl-2-picrylhydrazyl radical. BS-5 subfraction has shown strong antioxidant activities. The histopathological examination showed that the treatment of BS-5 subfraction has relieved the ballooning degeneration of hepatocytes which had been generated by CCl_4. It appears that the protective effect of BS-5 subfraction would be mediated of the attenuation of lipid peroxidation by acting as a free radical scavenger. which were based on the increase of superoxide dismutase activity.