De novo transcriptome profiling and characterization of voltage-sensitive sodium channel gene of Tropilaelaps mercedesae parasitizing honey bees

다국어 초록 (Multilingual Abstract)

Tropilaelaps mercedesae (Acari: Laelapidae) is an ectoparasite of the Western honey bee Apis mellifera and has become a major threat to the apiculture in Asia, including Korea. To establish gene resources of T. mercedesae, the whole transcriptome of mixed developmental stages of mites was analyzed by RNA sequencing. An mRNAfocused library was generated from total RNA extracted from the mixed stages (adults, nymphs and larvae) of T. mercedesae using the TruSeq RNA Library Preparation kit and sequenced using the HiSeq 2000 platform. A total of 59,041,198 reads were obtained with 85% Q30 value. Trimmed sequence data was de novo assembled into 64,868 contigs with an average sequence length of 878 bp. Transcriptome annotation to the NCBI nonredundant database resulted in a total of 14,336 Blast hits (22.1%). Functional analysis demonstrated that 11,919 contigs were grouped into 45 Gene Ontology (Conesa et al.) terms, and 1226 contigs were assigned to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Based on the transcriptome dataset, a large number of contigs associated with binding, cellular process and catalytic activity were identified. In order to provide basic information for efficient management of potential resistance to fluvalinate, a pyrethroid acaricide most widely used for the control of T. mercedesae, the gene encoding the voltage-sensitive sodium channel α-subunit (vssc), which is the molecular target of fluvalinate, was annotated and characterized

다국어 초록 (Multilingual Abstract)

Tropilaelaps mercedesae (Acari: Laelapidae) is an ectoparasite of the Western honey bee Apis mellifera and has become a major threat to the apiculture in Asia, including Korea. To establish gene resources of T. mercedesae, the whole transcriptome of mixed developmental stages of mites was analyzed by RNA sequencing. An mRNAfocused library was generated from total RNA extracted from the mixed stages (adults, nymphs and larvae) of T. mercedesae using the TruSeq RNA Library Preparation kit and sequenced using the HiSeq 2000 platform. A total of 59,041,198 reads were obtained with 85% Q30 value. Trimmed sequence data was de novo assembled into 64,868 contigs with an average sequence length of 878 bp. Transcriptome annotation to the NCBI nonredundant database resulted in a total of 14,336 Blast hits (22.1%). Functional analysis demonstrated that 11,919 contigs were grouped into 45 Gene Ontology (Conesa et al.) terms, and 1226 contigs were assigned to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Based on the transcriptome dataset, a large number of contigs associated with binding, cellular process and catalytic activity were identified. In order to provide basic information for efficient management of potential resistance to fluvalinate, a pyrethroid acaricide most widely used for the control of T. mercedesae, the gene encoding the voltage-sensitive sodium channel α-subunit (vssc), which is the molecular target of fluvalinate, was annotated and characterized.

참고문헌 (Reference)

1 정철의, "형태적 근거에 따른 국내 서식 중국가시응애 학명 Tropilaelaps mercedesea Anderson and Morgan, 2007 (Acari: Laelapidae) 변경" 한국양봉학회 29 (29): 217-221, 2014

2 Ramaswami, M., "Two sodium-channel genes in Drosophila: implications for channel diversity" 86 : 2079-2082, 1989

3 Bernard Vallat, A. T., "Terrestrial Animal Health Code. 24 ed"

4 Sammataro, D., "Parasitic mites of honey bees: life history, implications, and impact" 45 : 519-548, 2000

5 Thompson, C. E., "Parasite pressures on feral honey bees (Apis mellifera sp.)" 9 : 2014

6 Anderson, D. L., "Genetic and morphological variation of bee-parasitic Tropilaelaps mites (Acari: Laelapidae): new and re-defined species" 43 : 1-24, 2007

7 Rinkevich, F. D., "Diversity and convergence of sodium channel mutations involved in resistance to pyrethroids" 106 : 93-100, 2013

8 J. Marshall Clark, "Determination, mechanism and monitoring of knockdown resistance in permethrin-resistant human head lice, Pediculus humanus capitis" 한국응용곤충학회 12 (12): 1-7, 2009

9 Lee, M. L., "Density distribution of parasitic mites, Varroa destructor Anderson and Trueman and Tropilaelaps clarea Delfinado and Baker, on honeybee pupae (Apis mellifera L.) in autumn season in Korea" 20 : 6-, 2005

10 Forsgren, E., "Deformed wing virus associated with Tropilaelaps mercedesae infesting European honey bees (Apis mellifera)" 47 : 87-97, 2009

11 Lubinevski, Y., "Control of Varroa jacobsoni and Tropilaelaps clareae mites using Mavrik in Apis mellifera colonies under subtropical and tropical climates" 128 : 5-, 1988

12 Woyke, J., "Comparison of defense body movements of Apis laboriosa, Apis dorsata dorsata and Apis dorsata breviligula honey bees" 21 : 481-494, 2008

13 Huang, X., "CAP3: a DNA sequence assembly program" 9 : 868-877, 1999

14 Conesa, A., "Blast2GO:a universal tool for annotation, visualization and analysis in functional genomics research" 21 : 3674-3676, 2005

15 Akratanakul, P., "Beekeeping in Asia" United Nations Rome 1990