A Bacillus coagulans and its Application in Pine Pollen Fermentation

다국어 초록 (Multilingual Abstract)

In the study, a potential L-lactic acid-producing bacillus-strain LT1 was isolated. It was identified as Bacillus coagulans based on its morphological, physiological, biochemical properties, and phylogenetic similarity of 16S rDNA. The fermentation conditions of pine pollen by strain LT1 were optimized using Box-Behnken design and response surface methodology. Optimum values of the tested variables for production of viable counts were an inoculum size of 9.22%, temperature= 49.21℃, and an initial pH of 6.82. Under optimized conditions, the viable count was 5.49×109 CFU/mL.
Finally, the single factor experiment as preliminary research for fermentation product treatment was performed.

참고문헌 (Reference)

1 Saitou N, "The neighbor-joining method: A new method for reconstructing phylogenetic trees" 4 : 406-425, 1987

2 Cong T, "Study on the treatment of rats with benign prostatic hypertrophy by Masson Pine Pollen" 26 : 395-396, 2005

3 Liu X, "Study on the anti-fatigue function of pine pollen" 25 : 169-170, 2004

4 Jinguo W, "Stress resistance of lactic acid-producing Bacillus coagulans N001" 19 : 489-491, 2007

5 Lihua Y, "Spore-forming condition of Bacillus coagulants" 218 : 96-98, 2010

6 Nicholson WJ, "Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments" 64 : 548-572, 2000

7 Wei K, "Research on the effect of immune enhancement of Taishan pine pollen polysaccharide in murine" 43 : 3645-3652, 2010

8 Bai SM, "Protective effects of pine pollen on the gut microecology of rats under chronic stresses" 19 : 189-193, 2006

9 Zhang G, "Optimized ultrasonic-assisted extraction of flavonoids from Prunella vulgaris L. and evaluation of antioxidant activities in vitro" 12 : 18-25, 2011

10 Zhao WZ, "Optimized extraction of polysaccharides from corn silk by pulsed electric field and response surface quadratic design" 91 : 2201-2209, 2011

11 Shanyan Y, "Optimization of culture conditions for chromium-enriched protocorm of Dendrobium candidum" 14 : 1702-1706, 2013

12 Shanyan Yang, "Optimization of Pine Pollen Fermentation Conditions Using Lactobacillus paracasei" 한국식품과학회 24 (24): 155-160, 2015

13 Jian-Dong Cui, "Optimization of Medium on Exopolysaccharides Production in Submerged Culture of Cordyceps militaris" 한국식품과학회 19 (19): 1567-1571, 2010

14 Lane DJ, "Nucleic Acid Techniques in Bacterial Systematics" Wiley 371-375, 1991

15 Khan IA, "Leung's Encyclopedia of Common Natural Ingredients" John Wiley & Sons, Inc 83-, 2009

16 Tiina M, "L (+)-Lactic acid producer Bacillus coagulans SIM-7 DSM 14043 and its comparison with Lactobacillus delbrueckii ssp. Lactis DSM 20073" 39 : 861-867, 2006

17 Ljungh A, "Isolation, selection and characteristics of Lactobacillus paracasei subsp. paracasei F19" 14 : 4-6, 2002

18 Wenlong Z, "Isolation and identification of a Bacillus coagulans with stress resistance" 29 : 36-39, 2014

19 Xing H, "Isolation and characterization of a metsulfuron-methyl degrading bacterium Methylopila sp. S113" 60 : 152-158, 2007

20 Xiaoyan L, "Isolation and characterization of a bensulfuron-methyl degrading strain L1 of Bacillus" 20 : 111-119, 2010

21 He XY, "Effective components and pharmacological function of pine pollen" 35 : 78-80, 2007

22 Cazemier AE, "Effect of sporulation and recovery medium on the heat resistance and amount of injury of spores from spoilage bacilli" 90 : 761-770, 2001

23 Wang LX, "Determination of life elementin Yunnan pine pollen by microwave digestion and ICPAES" 4 : 102-104, 2007

24 Le Marrec C, "Bio-chemical and genetic characterization of coagulin, a new antilisterial bacteriocin in the pediocin family of bacteriocins, produced by Bacillus coagulans I4" 66 : 5213-5220, 2000

25 Holt JG, "Bergey’s Manual of Determinative Bacteriology" Lippincott Williams & Wilkins 529-551, 1994

26 Ripamonti B, "Administration of Bacillus coagulans in calves: Recovery from faecal samples and evaluation of functional aspects of spores" 33 : 991-1001, 2009