별 불가사리(Asterina pectinifera) 조직별 초산추출물의 생리활성 탐색 = Biological Activities of Acidic Extracts of the Starfish Asterina pectinifera

다국어 초록 (Multilingual Abstract)

The present study was performed to examine the contraction and relaxation responses of the smooth muscles, and search for antimicrobial and antioxidant activities in the tissues, of the starfish Asterina pectinifera . Frozen samples were extracted with distilled water containing 1% acetic acid. Extracts from all tissues showed potent antimicrobial activity against Escherichia coli D31. Relatively high levels of antimicrobial activity were also detected in the body extracts. Liver, tube feet, and body extracts caused contraction responses in the dorsal retractor muscles (DRM) of the starfish. In contrast, all tissues examined exhibited contractile activity in the esophagus of squid Todarodes pacificus . In addition, liver and gonad extracts caused contraction responses upon application to the intestine of the puffer fish Takifugu pardalis . Relaxation effects on the DRM of starfish were identified in most of the extracts, while no relaxant activity was detected in body extracts. Extracts from all tissues examined also exhibited antioxi- dant activities. The results of this study suggest that starfish are a potential source of novel bioactive compounds.

참고문헌 (Reference)

1 이현용, "불가사리(Asterias amurensis) 콜라겐 유래 저분자 펩타이드의 피부주름 억제활성" 한국식품과학회 39 (39): 625-629, 2007

2 이성태, "별불가사리 추출물의 면역세포 활성화 효과" 한국식품영양과학회 36 (36): 269-275, 2007

3 Lehrer RI, "Ultrasensitive assay for endogenous antimicrobial polypeptides" 137 : 167-173, 1991

4 Irwin DM, "Trout and chicken proglucagon: alternative splicing generates mRNA transcripts encoding glucagon-like peptide" 9 : 267-277, 1995

5 Hancock REW, "The role of cationic antimicrobial peptides in innate host defences" 8 : 402-410, 2000

6 Elphick MR, "The SALMFamides: a new family of neuropeptides in the starfish Asterias rubens" 198 : 2519-2525, 1991

7 Jensen J, "Substance-P-related and neurokinin- A related peptides from the brain of the cod and trout" 206 : 659-664, 1992

8 Erspamer V, "Structure and pharmacological actions of eledoisin, the active endecapeptide of the posterior salivary glands of Eledone" 18 : 58-59, 1962

9 Li C, "Strongylocins, novel antimicrobial peptides from the green sea urchin, Strongylocentrotus droebachiensis" 32 : 1430-1440, 2008

10 Chauvet J, "Special asvatocin and phasvatocin, two new oxytocinlike peptides in the spotted dogfish (Scyliorhinus caniculus)" 91 : 11266-11270, 1994

11 Dunlap WC, "Small-molecule antioxidants in marine organims : antioxidant activity of mycosporine-glycine" 112 : 105-114, 1995

12 Park JH, "Separation of antioxidant compounds from edible marine algae" 23 : 256-261, 1991

13 Kang DG, "Screening and comparison of antioxidant activity of solvent extracts of herbal medicines used in Korea" 87 : 231-236, 2003

14 Seo JK, "Purification of a novel arthropod defensin from the American oyster, Crassostrea virginica" 338 : 1998-2004, 2005

15 Ovchinnikova TV, "Purification and primary structure of two isoforms of arenicin, a novel antimicrobial peptide from marine polychaeta Arenicola marina" 577 : 209-214, 2004

16 Sampath Kumar NS, "Purification and identification of antioxidant peptides from the skin protein hydrolysate of two marine fishes, horse mackerel ( Magalaspis cordyla) and croaker (Otolithes ruber)" 42 : 1641-1649, 2012

17 Iijima N, "Purification and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major" 270 : 675-686, 2003

18 Seo JK, "Purification and antimicrobial function of ubiquitin isolated from the gill of Pacific oyster, Crassostrea gigas" 53 : 88-98, 2013

19 Fan J, "Purification and Identification of Antioxidant Peptides from Enzymatic Hydrolysates of Tilapia (Oreochromis niloticus) Frame Protein" 17 : 12836-12850, 2012

20 Iwakoshi E, "Protein Research Foundation" Protein Research Foundation 261-264, 1995

21 Martinage A, "Primary Structure of Histone H2A from Gonads of the Starfish Asterias rubens" 130 : 465-472, 1983

22 Destoumieux D, "Penaeidins, a new family of antimicrobial peptides isolated from the shrimp Penaeus Vannamei (Decapoda)" 272 : 28398-28406, 1997

23 Ames BN, "Oxidants, antioxidants, and the degenerative diseases of aging" 90 : 7915-7922, 1993

24 Elliott GR, "Neuroregulators: neurotransmitters and neuromodulators" 2 : 423-424, 1979

25 Krieger DT., "Neuropeptides in Neurologic and Psychiatric Disease" Raven Press 1-33, 1986

26 Elphick MR, "Neural control of muscle relaxation in echinoderms" 204 : 875-885, 2001

27 Mitta G, "Mytilin B and MGD2, two antimicrobial peptides of marine mussels: gene structure and expression analysis" 24 : 381-393, 2000

28 Mitta G, "Myticin, a novel cysteine-rich antimicrobial peptide isolated from haemocytes and plasma of the mussel Mytilus galloprovincialis" 265 : 71-78, 1999

29 Donia M, "Marine natural products and their potential applications as anti-infective agents" 3 : 338-348, 2003

30 Diaz-Miranda L, "Localization of the heptapeptide GFSKLYFamide in the sea cucumber Holothuria glaberrima (Echinodermata): a light and electron microscopic study" 352 : 626-640, 1995

31 Edward ER, "Invertebrate Zoology 7th edition" Thomson Learning 876-896, 2004

32 Charlet M, "Innate immunity. Isolation of several, cysteine- rich antimicrobial peptides from the blood of a mollusk, Mytilus edulis" 271 : 21808-21813, 1996

33 Park CH, "Hepcidin, a Urinary Antimicrobial Peptide Synthesized in the Liver" 276 : 7806-7810, 2001

34 Zadina JE, "Central nervous system effects of peptides, 1980-1985: a cross-listing of peptides and their central actions from the first six years of the journal peptides" 7 : 497-537, 1986

35 Krieger DT, "Brain peptides: what, where, and why?" 222 : 975-985, 1983

36 Matsunaga S, "Bioactive marine metabolites, Ⅳ. Isolation and the amino acid composition of discodermin A, an antimicrobial peptide, from the marine sponge Discodermia kiiensis" 48 : 236-241, 1985

37 Kim WG, "Benzastatin E, F, and G: new indoline alkaloids with neronal cell protectin activity form Streptomyces nitrosporesus" 53 : 4309-4316, 1997

38 Ovchinnikova TV, "Aurelin, a novel antimicrobial peptide from jellyfish Aurelia aurita with structural features of defensins and channel-blocking toxins" 348 : 514-523, 2006

39 Rice-Evans CA, "Antioxidant activities of flavonoids as bioactive compounds of food" 24 : 790-795, 1996

40 Miyata T, "Antimicrobial peptides, isolated from horseshoe crab hemocytes, tachyplesin II, and polyphemusins I and II : chemical structures and biological activity" 106 : 663-668, 1989

41 Koshino H, "Agrocybenine, novel class alkaloid from the Korean mushroom Agrocybe cylindracea" 37 : 4549-4550, 1996

42 Iwakiri M, "A novel oxytocin-like peptide isolated from the neural complexes of tunicate, Styele plicata" 7 : 1035-1041, 1990