環跳(GB30) 秦艽藥鍼이 신경병리성 통증 유발 흰쥐에 미치는 영향 = Effect of Gentianae Macrophyllae Radix Pharmacopuncture at Hwando (GB30) on Neuropathic Pain in Tibial and Sural Nerve Transected Rats

다국어 초록 (Multilingual Abstract)

Effect of Gentianae Macrophyllae Radix Pharmacopuncture at Hwando (GB30) on Neuropathic Pain in Tibial and Sural Nerve Transected Rats Ook Jae Lee1, Seon Wook Kim2 and Jeong Cheol Shin2,* 1Cham Joeun Hospital 2Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Dongshin University Objectives : The objective of this study was to investigate the effects of Gentianae Macrophyllae Radix pharmacopuncture (GP) at Hwando (GB30) in neuropathic pain induced rats.
Methods : Neuropathic pain in rats was induced by tibial and sural nerve transection. The rat subjects were divided into 6 groups : normal (Nor, n ＝ 5), control (Con, n ＝ 5), neuropathic pain- induced injected at GB30 with 1 ㎎/㎏ GP (GP-A, n ＝ 5), 5 ㎎/㎏ GP (GP-B, n ＝ 5) and 20 ㎎/㎏ GP (GP-C, n ＝ 5), and neuropathic pain-induced injected with 1㎎/㎏ Tramadol (Tramadol, n＝5). Injections were administered 2 times a week for a total of 5 treatments. After each treatment plantar withdrawal response was measured and after all 5 treatments were completed c-fos, Bax, Bcl-2, mGlu5 and leukocytes in the blood were analyzed.
Results : 1. Groups GP-A, GP-B and GP-C showed a meaningful decrease in the withdrawal response of mechanical allodynia compared to the control group.
2. Groups GP-A, GP-B and GP-C showed a meaningful decrease in the expression of c-fos compared to the control group.
3. Groups GP-A and GP-C showed a meaningful increase in the expression of mGluR5 compared to the control group.
4. Groups GP-A, GP-B and GP-C showed a meaningful decrease in Bax/Bcl-2 ratio compared to the control group.
Conclusion : These results suggest that Gentianae Macrophyllae Radix pharmacopuncture at Hwando (GB30) could decrease mechanical allodynia and could have analgesic and neuroprotective effects on the model of neuropathic pain.

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