Molecular Screening and Characterization of Antiviral Potatoes

다국어 초록 (Multilingual Abstract)

Potato plants carrying the Ry gene are extremely resistance to a number of potyviruses, but it is not known which variety expressed the resistance. In this investigation, combined classical and molecular techniques were used to identify virus resistance potatoes. Mechanical inoculation of 32 varieties of Korean potato cultivars, with potato virus Y (PVY), induced various symptoms, such as mosaic, yellow-ing, necrosis, mottle, vein clearing and vein bending. Different virus spreading patterns were observed, such as highly sensitive, moderate and resistant to PVYO inoculated leaves in different cultivars. From the results of double antibody sandwich-enzyme links immunosorbant assays (DAS-ELISA), coupled with reverse transcription polymerase chain reaction (RT-PCR), Winter valley and Golden valley were found to be highly susceptible and resistant cultivars to PVYO, respectively. TEM was used as a complementary method to conform the localization of the virus in leaf tissues. TEM detect virus particles in Golden val-ley; where, ELISA and RT-PCR were unable to detect the CP gene. However, the interior part of the tis-sues was severely deformed in PVYO infected Winter valley, than Golden valley. The Ry gene is involved in an induced response in PVYO infected Golden valley plants. The methods described in this study could be applied for the screening and development of antiviral potatoes.