벼 종자 기생성 Pseudomonas avenae와 P.glumae의 검출을 위한 PCR 방법에 의한 종자검정 = Rapid Seed Assay by PCR Method for Detecting Riceseedborne Pseudomonsa avenae and P.glumae

다국어 초록 (Multilingual Abstract)

Pseudomoas avenae and P. glumae were the causal pathogens of discoloration of rice seed in Korea. They caused the severe discoloration of rice seed thus affectted the quality and quantity of seeds. But similarities on symptom by them on the seeds and on morphology of phenotypically similar saprophytes on the media made the differenciation of them and seed assay difficult. To design, therefore, specific rapid seed assay for detecting and defferentiating them by PCR method, we selected optimal conditions, buffer, pH and extraction time, for buffer extraction and used the PCR method to rapidly identify the extracted and incubated each colony types. Incubation with 0.85% saline solution supplemented with Tween#20 (0.01%) for 2 hrs was selected as a optimal condition for extracting Pseudomonas spp. from seeds. Using this result, DNA from putative Pseudomonas spp. and saprophytes incubated on King's medium B was extracted and amplified with G1 and L1 primers. P. avenae and P. glumae showed characteristically polymorphic pattern, respectively, and saprophytes showed representative six types of completely different poly-morphic one.