상처 치유 응용을 위한 상피세포 증식 인자의 방출에 의한 가시광 경화형 Furfuryl Alginate 화합물의 재생 효과

국문 초록 (Abstract)

가시광 경화형 다당류 복합체는 합성되었고 상처 치료 응용을 위해 상피세포 증식 인자 (EGF)가 도입되었다. Alginate는 Furfurylamine과 함께 Furfuryl alginate (F-alginate)로 변형되었고, F-alginate는 가시광 반응을 위해 Rose Bengal과 섞어졌다. Furfuryl 작용기의 도입은 H-NMR과 UV/VIS spectroscopy를 통해 확인되었다. 가시광에 의한 가교는 micro-patterning과 무게 측정에 의해 평가되었다. 평윤도도 또한 측정하였다. 상처 치유 응용에서의 적용 가능성을 확인하기 위해, EGF는 F-alginate와 Rose Bengal의 혼합물에 함유되었다. F-alginate의 독성테스트와 mEGF 방출을 확인한 후, 세포 증식, 항 박테리아 활성, 동물 실험을 수행 하였다. 결론적으로 이러한 결과로부터, mEGF를 함유한 F-alginate는 상처 치유에 유용할 것으로 사료된다.

다국어 초록 (Multilingual Abstract)

A visible light-curable polysaccharide conjugate was prepared and was included with epidermal growth factor (EGF) for wound healing application. Alginate was modified with furfurylamine and the furfuryl alginate (F-alginate) was mixed with Rose Bengal for visible light-reactivity. The introduction of furfuryl group was confirmed by H-NMR and UV/VIS spectroscopy. Visible light-induced crosslinking was evaluated by micro-patterning and weight measurement. Swelling ratio was also determined. In order to verify its applicability in wound healing applications, EGF was included in the system of F-alginate and Rose Bengal. After the confirmation of no cytotoxicity of F-alginate and murine EGF release from the system, cell proliferation, anti-bacterial activity, and animal test were performed on the system. In conclusion, it was demonstrated that the system was useful for wound healing.

목차 (Table of Contents)

• 1. Introduction 1
• 2. Materials and Methods 5
• 2.1. Materials 5
• 2.2. Preparation and characterization of F-alginate 6
• 2.3. Observation of micro-patterning 8
• 2.4. Cross-linking ratio 8
• 2.5. Assay of mEGF 9
• 2.6. Cytotoxicity assay 10
• 2.7. Cell proliferation assay 11
• 2.8. In vitro Antibacterial assay 11
• 2.9. In vivo animal wound healing test 12
• 3. Results 14
• 3.1. Spectroscopic characterization 14
• 3.2. Micro-patterning 16
• 3.3. Cross-linking 17
• 3.4. Cytotoxicity of F-alginate 19
• 3.5. mEGF release 20
• 3.6. Cell proliferation 22
• 3.7. In vitro antibacterial activity 24
• 3.8 Wound healing and Histology 26
• 4. Discussion 30
• 5. Conclusion 34
• 6. References 35
• 7. 국문초록 42
• 8. Abstract 44
• 9. Acknowledgments 46