RISS 검색 - 국내학술지논문 상세보기

다국어 초록 (Multilingual Abstract)

Red-fl eshed radishes (RFRs) are economically important root vegetable in the Brassicaceae family that contain high concentrationsof radish-red pigment in their fl eshy root. However, the yield of the fl eshy root is limited and germplasm resourcesfor radish improvement are lacking. Genome doubling is an eff ective and effi cient breeding tool for the rapid creation ofnew germplasm resources. Therefore, we aimed to induce tetraploidy in RFRs to improve pigment production. RFR seedswere immersed in aqueous colchicine (0, 0.01, 0.05, 0.10, 0.15, and 0.20% (w/v)) for 24 h followed by cultivation at 25􁕑for 7 days, and those with uniform and distinctly swelled and short hypocotyls were selected as putative tetraploids. Theputative tetraploids were identifi ed by fl ow cytometry, and then we further examined the morphological and cytologicalcharacteristics of the diploid and tetraploid plants and measured gene expression via reverse transcription quantitative PCR.
The results indicated that the most suitable concentration of colchicine (0.05% (w/v)) could induce tetraploidy in RFRs, witha tetraploid-induction rate as high as 34.50%. In addition, tetraploids exhibited a “gigantism” eff ect in both morphologicaland cytological traits, including swelled hypocotyls, thicker cotyledons, larger stoma, wider and longer leaves, taller plants,larger fl owers, increased pollen size, larger seed pods and seeds, and a larger taproot. Moreover, compared with diploid plants,the pigment content and yield of pigment per plant of tetraploid plants were increased by 65.11% and 216.82%, respectively.
Therefore, we present a simple and effi cient method for tetraploid induction via soaking radish seeds in 0.05% (w/v) colchicinefor 24 h. The results showed that tetraploid plants not only exhibited obvious “gigantism” eff ects but also signifi cantlyenhanced accumulation of anthocyanins, and represent a practical breeding material for improving pigment yield in RFRs.

참고문헌 (Reference)

1 Mariana Cansian Sattler, "The polyploidy and its key role in plant breeding" Springer Science and Business Media LLC 243 (243): 281-296, 2016

2 Yavari S, "The eff ects of induction of autotetraploidy on the essential oil content and compositions of dragonhead (Dracocephalum moldavica L)" 14 : 484-489, 2011

3 Lv FS, "The characteristics of parents and seed production techniques of red radish “Yanzhihongyihao.”" 61 : 122-123, 2015

4 J. Christian Ambrose, "The Kinesin ATK5 Functions in Early Spindle Assembly inArabidopsis " Oxford University Press (OUP) 19 (19): 226-236, 2007

5 Yating Dong, "Salt tolerance diversity in diploid and polyploid cotton ( Gossypium ) species" Wiley 101 (101): 1135-1151, 2020

6 F. B. Chen, "Red Pigment Content and Expression of Genes Related to Anthocyanin Biosynthesis in Radishes (Raphanus sativus L.) with Different Colored Flesh" Canadian Center of Science and Education 8 (8): 126-135, 2016

7 Jan H. Schefe, "Quantitative real-time RT-PCR data analysis: current concepts and the novel “gene expression’s C T difference” formula" Springer Science and Business Media LLC 84 (84): 901-910, 2006

8 Feng-Quan Tan, "Polyploidy remodels fruit metabolism by modifying carbon source utilization and metabolic flux in Ponkan mandarin (Citrus reticulata Blanco)" Elsevier BV 289 : 110276-, 2019

9 Muhammad Zafar Iqbal, "Mysterious meiotic behavior of autopolyploid and allopolyploid maize" Pensoft Publishers 12 (12): 247-265, 2018

10 M. Landi, "Multiple functional roles of anthocyanins in plant-environment interactions" Elsevier BV 119 : 4-17, 2015