Antiproliferative and ameliorative effects of Tetracera potatoria and its constituent

다국어 초록 (Multilingual Abstract)

The effect of Tetracera potatoria leaves and its isolated constituent on polycystic ovarian syndrome (PCOS) and associated gynaecological cancers was investigated. Crude extract showed the lowest level of luteinizing hormone and the highest level of estradiol and follicle stimulating hormone, comparable to the normal control group. The hexane (IC50 = 34.8 ± 0.3 µg/mL), and dichloromethane (IC50 = 41.3 ± 0.8 µg/mL) fractions inhibited the proliferation of Chinese Hamster ovarian (CHO) cells. The dichloromethane fraction was subjected to purification using column chromatography, which led to the isolation of apigenin. The structure of the isolated compound was confirmed by the reported spectroscopic data. Apigenin inhibited the proliferation of both CHO and HeLa cells with an IC50 values of 22.2 ± 0.5 and 6.2 ± 0.6 µg/mL, respectively. The apigenin was isolated and reported for the first time in T. potatoria. The leaves extract of T. potatoria showed curative effect on irregular estrus cycle and hormonal imbalance. The isolated constituent showed anticancer potential, hence it could reduce the risk of gynaecological cancers among PCOS patients.

참고문헌 (Reference)

1 Sankaranarayanan R, "Worldwide burden of gynaecological cancer: the size of the problem" 20 (20): 207-225, 2006

2 Van Wagenen BC, "Ulosantoin, a potent insecticide from the sponge Ulosa ruetzleri" 58 (58): 335-337, 1993

3 Zhao L, "Treating infertility with the integration of traditional chinese medicine and assisted conception therapy" 6 (6): 1-7, 2011

4 Krstić J, "Transforming growth factor-beta and oxidative stress interplay: implications in tumorigenesis and cancer progression" 2015 (2015): 1-15, 2015

5 Dalzel JM, "The useful plants of west tropical Africa" Crown Agent Publication 1937

6 Burkill HM, "The useful plants of West Tropical Africa, vol l. Families AD (No. Ed. 2)" Royal Botanic Gardens 1985

7 Tao Ding, "The prevalence of polycystic ovary syndrome in reproductive-aged women of different ethnicity: a systematic review and meta-analysis" Impact Journals, LLC 8 (8): 96351-96358, 2017

8 Azziz R, "The prevalence and features of the polycystic ovary syndrome in an unselected population" 89 : 2745-2749, 2004

9 Adesanwo JK, "The effect of Tetracera potatoria Afzel and its constituent betulinic acid on gastric acid secretion and experimentally-induced gastric ulceration" 18 (18): 21-26, 2003

10 Shafiee MN, "The effect of Metformin on endometrial tumor-regulatory genes and systemic metabolic parameters in polycystic ovarian syndrome–a proof-of-concept study" 31 : 286-290, 2015

11 Lepley DM, "The chemopreventive flavonoid apigenin induces G2/M arrest in keratinocytes" 17 : 2367-2375, 1996

12 Fomogne-Fodjo MCY, "Secondary metabolites from Tetracera potatoria stem bark with anti-mycobacterial activity" 195 : 238-245, 2017

13 Miliauskas G, "Screening of radical scavenging activity of some medicinal and aromatic plant extracts" 85 : 231-237, 2004

14 Mensor LL, "Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method" 15 : 127-130, 2001

15 Gottschau M, "Risk of cancer among women with polycystic ovary syndrome: a danish cohort study" 136 : 99-103, 2015

16 Ziech D, "Reactive Oxygen Species (ROS)—induced genetic and epigenetic alterations in human carcinogenesis" 711 (711): 167-173, 2011

17 Mosmann T, "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays" 65 : 55-63, 1983

18 Yoshida M, "Quercetin arrests human leukemic T-cells in late G1 phase of the cell cycle" 52 (52): 6676-6681, 1992

19 Zangeneh FZ, "Psychological distress in women with polycystic ovary syndrome from Imam Khomeini Hospital Tehran" 13 (13): 1-6, 2012

20 Ugwu GO, "Prevalence, presentation and management of polycystic ovary syndrome in Enugu, south east Nigeria" 22 (22): 313-319, 2013

21 Bursal E, "Polyphenol contents and in vitro antioxidant activities of lyophilised aqueous extract of kiwifruit (Actinidia deliciosa)" 44 : 1482-1489, 2011

22 Ehrmann DA, "Polycystic ovary syndrome" 352 : 1223-1236, 2005

23 Adesanwo JK, "Phytochemical analysis and antioxidant activity of methanol extract and betulinic acid isolated from the roots of Tetracera potatoria" 6 : 903-907, 2013

24 Souza RP, "Oxidative stress triggered by apigenin induces apoptosis in a comprehensive panel of human cervical cancerderived cell lines" 2017 : 1-18, 2017

25 Hussain T, "Oxidative stress and inflammation: what polyphenols can do for us?" 2016 : 1-9, 2016

26 Gadducci A, "Metformin use and gynecological cancers: a novel treatment option emerging from drug repositioning" 105 : 73-83, 2016

27 Oliver-Bever B, "Medicinal plants in Nigeria" Nigerian College of Arts and Technology 1-138, 1960

28 Kafali H, "Letrozole-induced polycystic ovaries in the rat: a new model for cystic ovarian disease" 35 : 103-108, 2004

29 Avwioro OG, "Histochemistry and tissue pathology, principles and techniques" Claverianum 2010

30 Lian FR, "Genistein-induced G2-m arrest, P21WAF1 upregulation, and apoptosis in a non-small-cell lung cancer cell line" 31 (31): 184-191, 1998

31 Zhang C, "Flavonoids and phenolic compounds from Smilax scobinicaulis" 50 (50): 254-257, 2014

32 Ogunlakin AD, "Ethnobotanical survey of medicinal plants used as remedy for female infertility and menstrual disorder in Southwestern Nigeria" 15 : 205-217, 2020

33 Traganos F, "Effects of genistein on the growth and cell cycle progression of normal human lymphocytes and human leukemic MOLT-4 and HL-60 cells" 52 (52): 6200-6208, 1992

34 Sun J, "Effects of electro-acupuncture on ovarian P450arom, P450c17αand mRNA expression induced by letrozole in PCOS rats" 8 : 1-10, 2013

35 Kakadia N, "Effect of Vitex negundo L. seeds in letrozole induced polycystic ovarian syndrome" 9 (9): 336-345, 2018

36 Cardenas H, "Dietary apigenin exerts immune-regulatory activity in vivo by reducing NF-kappaB activity, halting leukocyte infiltration and restoring normal metabolic function" 17 (17): 323-339, 2016

37 Legro RS, "Diagnosis and treatment of polycystic ovary syndrome: an Endocrine Society clinical practice guideline" 98 : 4565-4592, 2013

38 Marcondes FK, "Determination of the estrous cycle phases of rats: some helpful considerations" 62 : 609-614, 2002

39 Murri M, "Circulating markers of oxidative stress and polycystic ovary syndrome (PCOS): a systematic review and meta-analysis" 19 (19): 268-288, 2013

40 Ting Hu, "Characterization of p53 in Chinese hamster cell lines CHO-K1, CHO-WBL, and CHL: implications for genotoxicity testing" Elsevier BV 426 (426): 51-62, 1999

41 Dumesic DA, "Cancer risk and PCOS" 78 : 782-785, 2013

42 Liu J, "Apigenin inhibits HeLa sphere-forming cells through inactivation of casein kinase 2alpha" 11 (11): 665-669, 2015

43 Nabavi MS, "Apigenin and breast cancers: from chemistry to medicine" 15 (15): 728-735, 2015

44 Choi JE, "Androgen receptor expression predicts decreased survival in early stage triple-negative breast cancer" 22 (22): 82-89, 2015

45 Woisky RG, "Analysis of propolis: some parameters and procedures for chemical quality control" 37 : 99-105, 1998

46 Betti JL, "An ethnobotanical study of medicinal plants among the Baka pygmies in the Dja biosphere reserve" 25 (25): 1-27, 2004

47 Nair AB, "A simple practice guide for dose conversion between animals and human" 7 (7): 27-31, 2016