발효 당용액 생산자원으로서 담수조류 그물말의 유용성 = Usefulness of Freshwater Alga Water-net (Hydrodictyon reticulatum) as Resources for Production of Fermentable Sugars

국문 초록 (Abstract)

본 연구에서는 담수녹조류인 그물말(Hydrodictyon reticulatum, HR)을 산업바이오 자원으로서 활용하는 방안을 강구하고자 일차적으로 효소당화의 용이성을 검토하였다. HR에 대하여 효소당화를 2% 고형분 함량에서 동일조건으로 수행했을 때, 다른 종류의 바이오매스(Spirulina, Chlorella, Scenedesmus, Cladophora, Corn stover)보다 glucose 수득율이 가장 높았다. HR을 분말화하지 않아도 최적조건의 효소처리량에서 당화가 모두 이루어지며, HR 당화용액의 citrate buffer strength가 0.1mM까지 낮아도 당수득율에 큰 지장이 없었다. 또한 HR을 고온의 전처리없이 실온상태에서 바로 당화시켜도 120℃ 처리에 비해 10% 미만의 당화율 감소만 나타내었다. 발효균주의 일반적인 생장적온인 37℃또는 pH 6.5에서도 당화가 정상적으로 잘 일어나 당화/발효를 동시에 진행시킬 수 있는 바이오매스로의 특징을 보였다. 효소량을 기준량의 1/10정도 줄여도 최적조건의 70~80%에 해당하는 glucose 수득율을 나타내었다. 그리고 본 실험조건에서 HR의 고형분 함량 10%까지 당수득율이 떨어지지 않았고 15%이상되어야 감소하기 시작하여 고농도 당용액 생산에도 좋은 특성을 나타내었다. 이들의 제반 결과는 HR이 당화가 매우 쉽게 일어나는 특징을 가진 조류 바이오매스임을 나타내준다. 이러한 장점뿐만 아니라 수집하기가 매우 용이한 사상조류(flilamentous algae)이기 때문에 다른 종류의 조류 바이오매스에 비해 바이오화학제품 생산을 위한 원료로서 향후 이용가치가 매우 높을 것으로 판단된다.

다국어 초록 (Multilingual Abstract)

To investigate the usefulness of freshwater alga Water-net (Hydrodictyon reticulatum, HR) as resources for production of fermentable sugars, the easiness of enzymatic saccharification was evaluated at first. When 6 plant materials (HR, Spirulina, Chlorella, Scenedesmus, Cladophora, Corn stover) were enzymatically hydrolyzed with 2% solid loading at the same condition, HR showed the highest ratio of saccharification based on glucose production. No milled HR was also completely saccharified at the amounts of optimal enzyme mixture. Glucose yield was not changed though the citrate buffer strength for saccharification was decreased from 0.1 M to 0.1 mM. Only about 10% yield reduction was observed compared to that of 120℃ treatment when HR was enzymatically hydrolyzed at room temperature. The saccharification was normally occurred at 37℃ and pH 6.5 which is general growth condition of fermentable microrganisms, suggesting that HR have a biomass characteristics applicable for the simultaneous saccharification and fermentation. The saccharification was occurred by more than 70~80% of one of the best condition although the supplied enzyme amounts was reduced to 1/10 volume. And the glucose yield by enzymatic hydrolysis was not decreased by 10% HR solid loading and began to decrease at more than 15% solid contents. Above these results show that HR is an interesting algal biomass which is relatively easy to be saccharified by hydrolyzing enzymes. In addition, HR is a flilamentous alga and very easy to be collected. Therefore, HR seems to be an useful and valuable resources in the economical production of fermentable sugars for manufacture of bio-chemical products.

목차 (Table of Contents)

• ABSTRACT 서언 재료 및 방법 결과 고찰 요약 인용문헌

참고문헌 (Reference)

1 윤병태, "구멍갈파래의 알칼리 과산화수소 전처리 및 효소 가수분해 특성" 한국공업화학회 22 (22): 336-339, 2011

2 Wyman, C. E., "What is (and is not) vital to advancing cellulosic ethanol" 25 (25): 153-157, 2007

3 Fitzgerald, G. P., "Use of potassium permanganate for control of problem algae" 58 (58): 609-614, 1966

4 Starý, J., "The uptake of ammonium, nitrite and nitrate ions by Hydrodictyonreticulatum" 15 (15): 193-198, 1987

5 Wells, R. D. S., "The rise and fall of water-net (Hydrodictyon reticulatum) in New Zealand" 37 : 49-54, 1999

6 Wi, S. G., "The potential value of the seaweed Ceylon moss (Gelidium amansii) as an alternative bioenergy resource" 100 : 6658-6660, 2009

7 Ge, L. P. Wang, "Study on saccharification techniques of seaweed wastes for the transformation of ethanol" 36 (36): 84-89, 2011

8 Wang, Z. H., "Studies on the ability of Hydrodictyon reticulatum to remove nitrogen and phosphorus under different environmental condition" 19 (19): 257-261, 1999

9 이성목, "Saccharomyces cerevisiae에 의한 Agar로부터 바이오 에탄올 생산" 한국공업화학회 20 (20): 290-295, 2009

10 Rai, U. N., "Removal of heavy metals from polluted waters by Hydrodictyon reticulatum (Linn.) Lagerheim" 87-88 : 509-515, 1989

11 Singh, A., "Removal of heavy metals from aqueous solution by common freshwater filamentous algae" 23 (23): 1115-1120, 2007

12 Aparicio Alonso, P. J., "Purification of surface waters using filamentous algae to absorb and recycle nutrients and/or heavy metals and generate biomass. Spain patent NO. ES 2251286"

13 Jeon, B. S., "Production process of monosaccharide and amino acid form seaweed by sub- and supercritical water hydrolysis. Patent No. KR 1057290"

14 Nguyen, C. M., "Production of L-lactic acid from a green microalga, Hydrodictyon reticulatum, by Lactobacillus paracasei LA104 isolated from the traditional Korean food, makgeolli" 110 : 552-559, 2012

15 Hendriks, A. T. W. M., "Pretreatments to enhance the digestibility of lignocellulosic biomass" 100 : 10-18, 2009

16 Alvira, P., "Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis：A review" 101 : 4851-4861, 2010

17 Lee, J. U., "Preparation method of biofuel from sea algae using irradiation. Patent No. KR 1083608"

18 Isa, A., "Preliminary study on ethanol production by using macro green algae" 88 : 912-917, 2009

19 Ghazala, B., "Phycochemistry and bioactivity of two microalgae (Volvocophyta) from Sindh" 1 (1): 343-350, 2004

20 Mata, T. M., "Microalgae for biodiesel production and other applications：A review" 14 (14): 217-232, 2010

21 John, R. P., "Micro and macroalgal biomass：A renewable source for bioethanol" 102 : 186-193, 2011

22 Fanyan, G. G., "Method of weed control in paddy fallow field of rice crop rotation. Patent No. RU 2330402"

23 Kim, G. S., "Method of producing hydrolysate from sea algae using acidic ionic liquid catalysts. Publication Patent No. KR 10-2010-0024665"

24 Kim, G. S., "Method of producing biofuel using sea algae. Publication Patent No. KR 10-2009-0025221"

25 Nyns, E. J., "Methane production by anaerobic digestion of algae"

26 Kang, D. H., "Liquefied extract of marine algae for producing bio-ethanol under high pressure and method for producing the same. Patent No. KR 0908425"

27 Hawes, I., "Influence of environmental factors on the growth in culture of a New Zealand strain of the fast-spreading alga Hydrodictyon reticulatum (water-net)" 5 (5): 437-445, 1993

28 Okuda, K., "Hydrothermal fractional pretreatment of sea algae and its enhanced enzymatic hydrolysis" 83 (83): 836-841, 2008

29 Zhou, D., "Hydrothermal Liquefaction of macroalgae Enteromorpha prolifera to bio-oil" 24 : 4054-4061, 2010

30 Nguyen, Minh Thu, "Hydrothermal Acid Pretreatment of Chlamydomonas reinhardtii Biomass for Ethanol Production" 한국미생물·생명공학회 19 (19): 161-166, 2009

31 Zhou, N., "Hydrolysis of Chlorella biomass for fermentable sugars in the presence of HCl and MgCl2" 102 (102): 10158-10161, 2011

32 Adams, J. M., "Fermentation study on Saccharina latissima for bioethanol production considering variable pre-treatments" 21 : 569-574, 2009

33 Hall, J., "Factors controlling the growth of field populations of Hydrodictyon reticulatum in New Zealand" 9 (9): 229-236, 1997

34 Appler, H. N., "Evaluation of Hydrodictyon reticulatum as protein source in feeds for Oreochromis (Tilapia) niloticus and Tilapia zillii" 27 (27): 327-334, 1985

35 Kim, N. J., "Ethanol production from marine algal hydrolysates using Escherichia coli KO11" 102 : 7466-7469, 2011

36 Ueno, Y., "Ethanol production by dark fermentation in the marine green alga, Chlorococcum littorale" 86 (86): 38-43, 1998

37 Choi, S. P., "Enzymatic pretreatment of Chlamydomonas reinhardtii biomass fro ethanol production" 101 : 5330-5336, 2010

38 Harun, R., "Enztmatic hydrolysis of microalgal biomass for bioethanol production" 168 (168): 1079-1084, 2011

39 Choi J. A., "Enhancement of fermentative bioenergy (ethanol/hydrogen) production using ultrasonication of Scenedesmus obliquus YSW15 cultivated in swine wastewater effluent" 4 : 3513-3520, 2011

40 Lee J. Y., "Comparison of several methods for effective lipid extraction from microalgae" 101 (101): 75-77, 2010

41 NREL, "Chemical analysis and testing standard procedure, no. 001-014"

42 Harun, R., "Bioprocess engineering of microalgae to produce a variety of consumer products" 14 : 1037-1047, 2010

43 Scott, S. A., "Biodiesel from algae：challenges and prospects" 21 (21): 277-286, 2010

44 Legros, A., "An integrated system： mass algae culture in polluted luke-warm water for production of methane, high-value products and animal feed"