Induction of Genetic Variants of Horticultural Crops by DNA Injection, Protoplasts and Embryo Culture = DNA주입, 원형질체 및 배 배양을 통한 원예작물의 유전자 변이유도

다국어 초록 (Multilingual Abstract)

Genetic transformation was induced by microinjection and co-cultivation techniques. Total DNA extracted from Altari(A) radish leaves was microinjected into the ovary of Kungzung(K) radish when the recipient was self-pollinated. As judged by their hypocotyl colors and leaf types. seven plants out of 2,760 were found transformed when the seeds harvested from the treated K plants were grown(TS-0 generation). When the seeds from those 7 transformants were grown again (Ts-1 generation), the hypocotyl colors, the number of parted leaflets, and leaf margins were segregated to the ratio of 3 to 1 as if the TS-1 plants were the hybrids between A adn K. The ratio suggested that each of such characters was determined by a single gene, and those genes were stably incorporated into the recipient genome. The transformation was also demonstrated at the seed stage by comparing the banding patterns of not only total proteins but the isozymes of malate dehydrogenase, urease, and acid phosphatase. Some transformants have been obtained by co-cultivating immature K embryos with the DNA prepared from A leaves. The optimum conditions have been established to culture tomato protoplasts in the presence of potato leaf DNA. Production of genetic variants in this study implies that such techniques could be a way to bypass the vector system in transformation studies and the genetic barrier could be overcome at the molecular level.