Microbial Community of Healthy Thai Vegetarians and Non-Vegetarians, Their Core Gut Microbiota, and Pathogen Risk = Microbial Community of Healthy Thai Vegetarians and Non-Vegetarians, Their Core Gut Microbiota, and Pathogen Risk

다국어 초록 (Multilingual Abstract)

Pyrosequencing analysis of intestinal microflora from healthy Thai vegetarians and nonvegetarians exhibited 893 OTUs covering 189 species. The strong species indicators of vegetarians and non-vegetarians were Prevotella copri and Bacteroides vulgatus as well as bacteria close to Escherichia hermanii with % relative abundance of 16.9 and 4.5-4.7, respectively. Core gut microbiota of the vegetarian and non-vegetarian groups consisted of 11 and 20 different bacterial species, respectively, belonging to Actinobacteria, Firmicutes, and Proteobacteria commonly found in both groups. Two species, Faecalibacterium prausnitzii and Gemmiger formicilis, had a prevalence of 100% in both groups. Three species, Clostridium nexile, Eubacterium eligens, and P. copri, showed up in most vegetarians, whereas more diversity of Collinsella aerofaciens, Ruminococcus torques, various species of Bacteroides, Parabacteroides, Escherichia, and different species of Clostridium and Eubacterium were found in most nonvegetarians. Considering the correlation of personal characters, consumption behavior, and microbial groups, the age of non-vegetarians showed a strong positive correlation coefficient of 0.54 (p = 0.001) to Bacteroides uniformis but exhibited a moderate one to Alistipes finegoldii and B. vulgatus. Only a positive moderate correlation of body mass index and Parabacteroides distasonis appeared. Based on the significant abundance of potential pathogens, the microbiota of the non-vegetarian group showed an abundance of potential pathogen varieties of Bilophila wadsworthia, Escherichia coli, and E. hermannii, whereas that of the vegetarian group served for only Klebsiella pneumoniae. These results implied that the microbiota of vegetarians with high abundance of P. copri and low potential pathogen variety would be a way to maintain good health in Thais.

참고문헌 (Reference)

1 Nair P, "Vegetarianism, dietary fibre and gastro-intestinal disease" 12 : 177-185, 1994

2 An QL, "Using confocal laser scanning microscope to visualize the infection of rice root by GFP-labelled Klebsiella oxytoca SA2, an endophytic diazotroph" 43 : 558-564, 2001

3 Nakayama J, "Up to the species-level community analysis of human gut microbiota by 16S rRNA amplicon pyrosequencing" 32 : 69-76, 2013

4 Tonstad S, "Type of vegetarian diet, body weight, and prevalence of type 2diabetes" 32 : 791-796, 2009

5 Duncan SH, "The role of pH in determining the species composition of the human colonic microbiota" 11 : 2112-2122, 2009

6 Clemente JC, "The impact of the gut microbiota on human health: an integrative view" 148 : 1258-1270, 2012

7 Doe J, "The human intestinal microbiota" 34 : S7-S15, 2010

8 Vincent WF, "The genus Escherichia - an overview" 20 : 36-48, 2013

9 Ottman N, "The function of our microbiota: who is out there and what do they do" 2 : 1-11, 2012

10 Mariat D, "The Firmicutes/Bacteroidetes ratio of the human microbiota changes with age" 9 : 123-, 2009

11 Kim MS, "Strict vegetarian diet improves the risk fators associated with metabolic diseases by modulating gut microbiota and reducing intestinal inflammation" 5 : 765-775, 2013

12 Supatjaree Ruengsomwong, "Senior Thai Fecal Microbiota Comparison Between Vegetarians and Non-Vegetarians Using PCR-DGGE and Real-Time PCR" 한국미생물·생명공학회 24 (24): 1026-1033, 2014

13 Guerrant RL, "Role of toxigenic and invasive bacteria in acute diarrhea of childhood" 293 : 567-571, 1975

14 Gentschew L, "Role of nutrition and microbiota in susceptibility to inflammatory bowel diseases" 56 : 524-535, 2012

15 Kadner RJ, "Repression of synthesis of the vitamin B 12receptor in Escherichia coli" 136 : 1050-1057, 1978

16 Nakayama J, "Pyrosequence-based 16S rRNA profiling of gastrointestinal microbiota" 29 : 83-96, 2010

17 Lathrop SK, "Peripheral education of the immune system by colonic commensal microbiota" 478 : 250-254, 2011

18 Kaper JB, "Pathogenic Escherichia coli" 2 : 123-140, 2004

19 Cruz AY, "Pantoea agglomerans, a plant pathogen causing human disease" 45 : 1989-1992, 2007

20 Jain A, "Pantoea agglomerans infection behaving like a tumor after plant thorn injury: an unusual presentation" 55 : 386-388, 2012

21 Veshnyakova A, "On the interaction of Clostridium perfringens enterotoxin with claudins" 2 : 1336-1356, 2010

22 Martínez I, "Long-term temporal analysis of the human fecal microbiota revealed a stable core of dominant bacterial species" 8 : e69621-, 2013

23 Wu GD, "Linking long-term dietary patterns with gut microbial enterotypes" 334 : 105-108, 2011

24 Ladha JK, "Isolation and identification of nitrogen-fixing Enterobacter clocae and Klebsiella planticola associated with rice plants" 29 : 1301-1308, 1983

25 Woodmansey EJ, "Intestinal bacteria and aging" 102 : 1178-1186, 2007

26 De Filippo C, "Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa" 107 : 14691-14696, 2010

27 Chelius MK, "Immunolocalization of dinitrogenase reductase produced by Klebsiella pneumoniae in association with Zea mays L" 66 : 783-787, 2000

28 Yatsuneko T, "Human gut microbiome viewed across age and geography" 486 : 222-227, 2012

29 Meer RR, "Human disease associated with Clostridium perfringens enterotoxin" 150 : 75-94, 1997

30 De Filippis F, "High-level adherence to a Mediterranean diet beneficially impacts the gut microbiota and associated metabolome" 2015

31 Bagley ST, "Habitat association of Klebsiella species" 6 : 52-56, 1985

32 Claesson MJ, "Gut microbiota composition correlates with diet and health in the elderly" 488 : 178-184, 2012

33 Crowe FL, "Fruit and vegetable intake and mortality from ischaemic heart disease: results from the European Prospective Investigation into Cancer and Nutrition (EPIC)-Heart Study" 465 : 1-9, 2011

34 Hayashi H, "Fecal microbial diversity in a strict vegetarian as determined by molecular analysis and cultivation" 46 : 819-831, 2002

35 Sokol H, "Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients" 43 : 16731-16736, 2008

36 Miquel S, "Faecalibacterium prausnitzii and human intestinal health" 16 : 1-7, 2013

37 Kabeerdoss J, "Faecal microbiota composition in vegetarians: comparison with omnivores in a cohort of young women in southern India" 108 : 953-957, 2012

38 Poulou A, "Escherichia hermannii as the sole isolate from a patient with purulent conjunctivitis" 46 : 3848-3849, 2008

39 Arumugam M, "Enterotypes of the human gut microbiome" 473 : 174-180, 2011

40 Ananthan S, "Enterotoxigenicity of Klebsiella pneumoniae associated with childhood gastroenteritis in Madras, India. Jpn" 52 : 16-17, 1999

41 Reiter B, "Endophytic nifH gene diversity in African sweet potato" 49 : 549-555, 2003

42 Jeffery IB, "Diet-microbiota interactions and their implications for healthy living" 5 : 234-252, 2013

43 Wu GD, "Diet, the human gut microbiota, and IBD" 24 : 117-120, 2013

44 David LA, "Diet rapidly and reproducibly alters the human gut microbiome" 505 : 559-563, 2014

45 Martinez L, "Diazotrophic bacteria associated with banana (Musa spp.)" 257 : 35-47, 2003

46 Dong YM, "Comparison between two Klebsiella: the plant endophyte K. pneumoniaee 342 and a clinical isolate, K. pneumoniaee MGH78578" 35 : 247-259, 2003

47 Nam Y, "Comparative analysis of Korean human gut microbiota by barcoded pyrosequencing" 6 : e22109-, 2011

48 Knittel MD, "Colonization of the botanical environment by Klebsiella isolates of pathogenic origin" 34 : 557-563, 1977

49 Pien FD, "Colonization of human wounds by Escherichia vulneris and Escherichia hermannii" 22 : 283-285, 1985

50 Bentley R, "Biosynthesis of vitamin K (menaquinone) in bacteria" 46 : 241-280, 1982

51 Brenner DJ, "Atypical biogroups of Escherichia coli found in clinical specimens and description of Escherichia hermannii sp. nov" 15 : 703-713, 1982

52 Zhang HJ, "Attenuated associations between increasing BMI and unfavorable lipid profiles in Chinese Buddhist vegetarians" 22 : 249-256, 2013

53 Matijaši BB, "Association of dietary type with fecal microbiota in vegetarians and omnivores in Slovenia" 53 : 1051-1064, 2013

54 Palus JA, "Adiazotrophic bacterial endophyte isolated from stems of Zea mays L., and Zea luxurians Itlis and Doebley" 186 : 135-142, 1996