Comparative Studies on Cotton Seed Germinability with Tetrazolium Viability Test and X-ray Contrast Methods

다국어 초록 (Multilingual Abstract)

Seed viability testing provides valuable information for assessing seed lot germinability. However, most testing methods require destruction of seed prior to test. Because the dissected seeds for viability test cannot be used further evaluation, the nondestructively X-ray photography technique that can be applied for the evaluation of seed quality has been developed. In order to know the validity and accuracy of X-ray photography technique in seed evaluation test that conducted to remove the abnormal seed from a seed lot, we have compared the results from tetrazolium viability test, germination test and X-ray contrast method in cotton. Metallic salts treatment increased the efficiency of X-ray photographic method by enhancing the penetration of X-ray in abnormal or damaged seeds rather than normal seeds that have strong and well-organized tissues in seed. Cotton seeds presoaked for 16 hr in distilled water followed by soaking into metallic salt solution (5% NaI in water) for 60 min were easily classified seeds into dead seed and viable seed based on the radiography images obtained by X-ray radiation. We concluded that soft X-ray photography was reliable to find out the various defective characters due to heat and mechanical damage of seeds.

참고문헌 (Reference)

1 Simak, M., "X-ray photography and sensitivity in forest tree species" 39 : 458-468, 1953

2 Swaminathan, M. S., "X-ray analysis of the anatomy and viability of seeds of some economic plants" 21 : 129-135, 1961

3 Kamra, S. K., "Use of X-ray radiography for studying seed quality in tropical forestry" 131 : 1-34, 1976

4 Kim, O. R., "Use of Soft X-ray photography for studying seed quality in grass and tree" 55 : 47-54, 1982

5 Kim, S. H., "The use of multiple tests in predicting the vigor of soybean seeds" 32 : 268-276, 1987

6 Argerich, C. A., "The effects of priming and ageing on seed vigour in tomato" 40 : 599-607, 1989

7 Simak, M., "The X-ray contrast method for seed testing: Scots pine-Pinus silvestris" 47 (47): 1-22, 1957

8 Bulant, H., "Test Instruction according to the Lakon topographical tetrazolium method for determine germination capacity of tree seeds" 22 (22): 1-4, 1959

9 Yates, H. O., "Radiography for detection and study of insects in plant seeds" 65-78, 1974

10 Smith, A. J., "Radiographic density measurements for determination of viability and vigor in corn (Zea may L.) seeds" 13 : 759-768, 1985

11 Devillez, F., "Quelque aspects morphologique de la germination chez Pseudotsuga menziesii(Mirb. )Franco var. Menziesii" 69 : 27-44, 1971

12 Van der Burg, W. J., "Prediction of tomato (Lycopersicon esculentum Mill.) seedling morphology by X-ray analysis of seeds" 119 (119): 258-263, 1994

13 Simak, M., "New used of x-ray method for the analysis of forest seed" 1970

14 Kim, S. H., "Multiple seed vigour indices to predict field emergence and performance of barley" 22 : 59-68, 1994

15 MSTATC, "Michigan State University MSTAT Development Team. MSTAT User’s Guide"

16 Simak, M., "Kottfore komst och frovarde hos gran och tall insamlingssasongen 1973-74" 60 : 1973

17 ISTA, "International Rules for Seed Testing" International Seed Testing Association 2007

18 ISTA, "ISTA Working Sheets on Tetrazolium Testing I" International Seed Testing Association 2003

19 Lakon, G., "Die Fsetstellung der keim fahigkeit der koniferensamen nach dem topographischen Tetrazolium- Verfahren" 4 : 83-87, 1950

20 Kamra, S. K., "Determination of germinability of cucumber seed with X-ray contrast method" 29 (29): 519-534, 1964

21 Chen. P., "A review of non-destructive methods for quality evaluation and sorting of agricultural products" 49 : 85-98, 1991