Characterization of Blackberry Extract and Its Antiproliferative and Anti-Inflammatory Properties

다국어 초록 (Multilingual Abstract)

Blackberries are rich in polyphenols, including anthocyanins. Polyphenols are hypothesized to have biologi-cal activities that may impact positively on human health. In these studies, an anthocyanin-rich extract from Hull blackber-ries grown in Kentucky was obtained and fully characterized in terms of total anthocyanin and phenolic content, polymericcolor, anthocyanin composition, and total antioxidant capacity. In vitrocell culture studies showed that the blackberry extractinhibited HT-29 colon tumor cell growth in a concentration-dependent manner with 49.2 .g of total anthocyanins/mL in-hibiting HT-29 cell growth up to 66% at 72 hours. Likewise, in a concentration-dependent manner, total anthocyanin con-centrations in the range of 040 .g/mL suppressed both high-dose (10 .g/mL) and low-dose (0.1 .g/mL) lipid A-inducedinterleukin-12 release from mouse bone marrow-derived dendritic cells. These results suggest that Hull blackberry extract(HBE) has potent antioxidant, antiproliferative, and anti-inflammatory activities and that HBE-formulated products may havethe potential for the treatment and/or prevention of cancer and/or other inflammatory diseases.

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