우고닌(Wogonin)이 poly I:C로 유발된 TM4세포 내 하이드로겐퍼록사이드 생성에 미치는 영향 = Effect of Wogonin on Intracellular Hydrogen Peroxide Production of TM4 Mouse Sertoli cells stressed with polyinosinic:polycytidylic acid

다국어 초록 (Multilingual Abstract)

Objectives : The aim of this study is to investigate the effect of wogonin on the production of hydrogen peroxide in polyinosinic:polycytidylic acid (poly I:C)-stimulated TM4 mouse sertoli cells.
Methods : TM4 were treated with poly I:C (50 ug/mL) and wogonin at concentrations of 5, 10, 25, and 50 μM for 30 min, 2 hr, 12 hr, 18 hr, and 24 hr. The production of intracellular hydrogen peroxide was measured by dihydrorhodamine 123 assay.
Results : For 30 min, 2 hr, 12 hr, 18 hr, and 24 hr treatment, wogonin significantly inhibited intracellular hydrogen peroxide productions of TM4 at the concentration of 5, 10, 25, and 50 μM (p <0.05). In details, production of hydrogen peroxide in poly I:C-stimulated TM4 treated for 30 min with wogonin at concentrations of 5, 10, 25, and 50 μM was 95.67%, 92.69%, 92.05%, and 91.97% of the control group treated with poly I:C only, respectively; the production of hydrogen peroxide for 2 hr was 94.44%, 94.41%, 93%, and 92.98%, respectively; production of hydrogen peroxide for 12 hr was 96.78%, 95.32%, 94.33%, and 93.17%, respectively; production of hydrogen peroxide for 18 hr was 94.7%, 93.4%, 93.38%, and 93.35%, respectively; and production of hydrogen peroxide for 24 hr was 95.75%, 94.77%, 94.58%, and 92.8%, respectively.
Conclusions : Wogonin might have anti-viral property related with its inhibition of intracellular hydrogen peroxide production in poly I:C-stimulated TM4 cells.

참고문헌 (Reference)

1 박완수, "마우스 대식세포(Raw 264.7)에 대한 한약조성물 KOCO-P1의 세포활성 연구" 대한본초학회 23 (23): 151-157, 2008

2 박완수, "黃芩 물추출물이 마우스 대식세포의 hydrogen peroxide 생성에 미치는 영향" 대한본초학회 26 (26): 53-58, 2011

3 Wang Z, "scRNA-seq Profiling of Human Testes Reveals the Presence of the ACE2 Receptor, A Target for SARS-CoV-2 Infection in Spermatogonia, Leydig and Sertoli Cells" 9 (9): 920-, 2020

4 Khan NM, "Wogonin, a plant derived small molecule, exerts potent anti-inflammatory and chondroprotective effects through the activation of ROS/ERK/Nrf2 signaling pathways in human Osteoarthritis chondrocytes" 106 : 288-301, 2017

5 Guo X, "Wogonin preventive impact on hippocampal neurodegeneration, inflammation and cognitive defects in temporal lobe epilepsy" 27 (27): 2149-2156, 2020

6 Hong M, "Wogonin inhibits cell cycle progression by activating the glycogen synthase kinase-3 beta in hepatocellular carcinoma" 68 : 153174-, 2020

7 Yang D, "Wogonin induces cellular senescence in breast cancer via suppressing TXNRD2 expression" 94 (94): 433-447, 2020

8 Fan L, "Wogonin Suppresses IL-10 Production in B Cells via STAT3 and ERK Signaling Pathway" 2020 : 032425-, 2020

9 Koh H, "Wogonin Influences Osteosarcoma Stem Cell Stemness Through ROS-dependent Signaling" 34 (34): 1077-1084, 2020

10 Wang Y, "Wogonin Induces Apoptosis and Reverses Sunitinib Resistance of Renal Cell Carcinoma Cells via Inhibiting CDK4-RB Pathway" 11 : 1152-, 2020

11 Zheng ZC, "Wogonin Ameliorates Renal Inflammation and Fibrosis by Inhibiting NF-κB and TGF-β1/Smad3 Signaling Pathways in Diabetic Nephropathy" 14 : 4135-4148, 2020

12 Khan S, "Wogonin Alleviates Hyperglycemia Through Increased Glucose Entry into Cells Via AKT/GLUT4 Pathway" 25 (25): 2602-2606, 2019

13 Zhuang J, "Wogonin Accelerates Hematoma Clearance and Improves Neurological Outcome via the PPAR-γ Pathway After Intracerebral Hemorrhage" 12 (12): 660-675, 2021

14 Fortier ME, "The viral mimic, polyinosinic : polycytidylic acid, induces fever in rats via an interleukin-1-dependent mechanism" 287 (287): R759-R766, 2004

15 Shen Q, "The ACE2 expression in Sertoli cells and germ cells may cause male reproductive disorder after SARS-CoV-2 infection" 24 (24): 9472-9477, 2020

16 Yang CS, "TLR3-triggered reactive oxygen species contribute to inflammatory responses by activating signal transducer and activator of transcription-1" 190 (190): 6368-6377, 2013

17 Li Y, "TLR3 ligand Poly IC Attenuates Reactive Astrogliosis and Improves Recovery of Rats after Focal Cerebral Ischemia" 21 (21): 905-913, 2015

18 Kang SS, "Staphylococcus aureus lipoproteins augment inflammatory responses in poly I : C-primed macrophages" 111 : 154-161, 2018

19 Katoh S, "Preventive effect of galectin-9 on doublestranded RNA-induced airway hyperresponsiveness in an exacerbation model of mite antigen-induced asthma in mice" 39 (39): 453-462, 2013

20 Huynh DL, "Potential therapeutic and pharmacological effects of Wogonin : an updated review" 47 (47): 9779-9789, 2020

21 Sharma D, "Poly(I : C)Priming Exacerbates Cecal Ligation and Puncture-Induced Polymicrobial Sepsis in Mice" 41 (41): 328-336, 2018

22 박완수, "Oroxylin A가 polyinosinic-polycytidylic acid로 유발된 생쥐 서톨리세포 TM4의 hydrogen peroxide 생성증가에 미치는 영향" 한의병리학회 28 (28): 384-389, 2014

23 Wang L, "Neuroprotective effect of Wogonin on Rat's brain exposed to gamma irradiation" 204 : 111775-, 2020

24 Xia QD, "Network pharmacology and molecular docking analyses on Lianhua Qingwen capsule indicate Akt1 is a potential target to treat and prevent COVID-19" 53 (53): 12949-, 2020

25 박완수, "Lipopolysaccharide로 활성화된 마우스 대식세포에서 艾葉 물추출물의 면역활성 연구" 대한본초학회 24 (24): 151-157, 2009

26 Crow JP, "Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species" 1 (1): 145-157, 1997

27 Khan S, "Can Wogonin be Used in Controlling Diabetic Cardiomyopathy" 25 (25): 2171-2177, 2019

28 Hui KM, "Anxiolytic effect of wogonin, a benzodiazepine receptor ligand isolated from Scutellaria baicalensis Georgi" 64 (64): 1415-1424, 2002

29 Lee JY, "Anti-Inflammatory Effect of Wogonin on RAW 264.7 Mouse Macrophages Induced with Polyinosinic-Polycytidylic Acid" 20 (20): 6888-6900, 2015

30 Sawicki SG, "A new model for coronavirus transcription" 440 : 215-219, 1998