<i>Nocardioides daecheongensis</i> sp. nov., isolated from soil

다국어 초록 (Multilingual Abstract)

<P>Strain KIS2-16<SUP>T</SUP> was isolated from a soil sample collected from Daecheong Island of Incheon region, South Korea. KIS2-16<SUP>T</SUP> was Gram-staining-positive, aerobic, non-spore-forming, non-motile, catalase-positive, oxidase-negative and mesophilic. On the basis of 16S rRNA gene sequence analysis, strain KIS2-16<SUP>T</SUP> represented a member of the genus <I>Nocardioides</I>, being most closely related to the type strains of species of the genus <I>Nocardioides</I>, <I>Nocardioides maradonensis</I> RP-B30<SUP>T</SUP> (97.8 % sequence similarity) and <I>Nocardioides ultimimeridianus</I> RP-B26<SUP>T</SUP> (97.0 %). The fatty acid profile of KIS2-16<SUP>T</SUP> was dominated by C<SUB>18 : 1</SUB>ω9<I>c</I>, C<SUB>17 : 1</SUB>ω8<I>c</I>, C<SUB>16 : 0</SUB>, C<SUB>18 : 0</SUB> 10-methyl (TBSA), C<SUB>16 : 0</SUB> 2-OH and C<SUB>17 : 0</SUB> 2-OH. The major isoprenoid quinone was MK-8(H<SUB>4</SUB>), and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The peptidoglycan structure was A3γ-type with <SMALL>ll</SMALL>-diaminopimelic acid. The genomic DNA G+C content of KIS2-16<SUP>T</SUP> was 64.9 mol%. Strain KIS2-16<SUP>T</SUP> showed DNA–DNA hybridization values of less than 70 % with the closely related species of the genus <I>Nocardioides</I>. Based on phenotypic, genotypic and phylogenetic data, the isolate represents a novel species of the genus <I>Nocardioides</I>, for which the name <I>Nocardioides daecheongensis</I> sp. nov. (type strain KIS2-16<SUP>T</SUP> = DSM 27136<SUP>T</SUP> = KACC 17297<SUP>T</SUP> = NBRC 109597<SUP>T</SUP>) is proposed.</P>