문화재로부터 선별된 미생물의 동정 및 바이오매스분해 활성 확인 = Identification of Microorganisms Isolated from Ancient Cultural Heritage and Verification of Biomass Degrading Activities

다국어 초록 (Multilingual Abstract)

Ancient cultural heritage is very important in historicalworth which they must be preserved in. However, theyare rotten and disintegrated easily by various microorganismslike fungi and bacteria because they are mostly made up ofwood and paper. In order to control these microbes, we needto investigate these microbes to maintain historical worth ofancient cultural heritage. We collected several samples fromsurface of ancient documents and wooden artifacts. And thenmicroorganisms were isolated from the samples and identifiedthrough sequencing of 16S ribosomal RNA or internaltranscribed spacer region. As results of sequencing, two bacterialstrains (E-1, O-1) were identified as Staphylococcushominis and S. epidermidis and four fungal strains (A-1, M-1,M-2, K-1) were identified as Schizophyllum commune, Peniophoraincarnata, Cephalotheca foveolata, Aspergillus niger.
Among them, A-1 strain showed relatively higher celluloseand CMC degrading activities and K-1 strain showed relativelyhigher xylan degrading activity than other strains.

참고문헌 (Reference)

1 윤영미, "흰 코뿔소 배설물로부터 분리한 Bacillus pumilus H10-1의 Xylanase 활성" 한국생물공학회 29 (29): 316-322, 2014

2 김시현, "수계에 인접한 목조건축물의 생물분포 모니터링 연구 - 여주 신륵사를 중심으로 -" 한국문화재보존과학회 33 (33): 267-274, 2017

3 서민석, "목조문화재 흰개미 서식환경 특성 연구" 한국문화재보존과학회 31 (31): 387-393, 2015

4 이민영, "공주 송산리 고분 내 미생물 분포 및 보존환경 연구" 한국문화재보존과학회 30 (30): 169-179, 2014

5 문현준, "건축물 내 습기 및 미생물 제어를 위한 건축 자재의 열·습기 물성 측정 및 적용" 대한건축학회 28 (28): 227-234, 2012

6 서민석, "강진 무위사 성보박물관 전시실의 미생물 서식환경 특성 연구" 한국문화재보존과학회 29 (29): 333-343, 2013

7 Bastian, F., "The microbiology of lascaux cave" 156 : 644-652, 2010

8 Kang, S. W., "Production of cellulase and xylanase by Aspergillus niger KKS" 4 : 49-55, 1994

9 Ahmed Imtiaj, "Physicochemical Requirement for the Vegetative Growth of Schizophyllum commune Collected from Different Ecological Origins" 한국균학회 36 (36): 34-39, 2008

10 Romualdus N. C. Utomo, "Phanerochaete chrysosporium의 고상발효를 통한 리그노셀룰로오즈 분해 및 당화" 한국생물공학회 32 (32): 96-102, 2017

11 차영록, "Paenibacillus jamilae BRC15-1의 Cellulase 생산 최적화" 한국생물공학회 30 (30): 283-290, 2015

12 Suh, S. O., "Molecular phylogeny of the cleistothecial fungi placed in Cephalothecaceae and Pseudeurotiaceae" 91 : 836-848, 1999

13 Kloos, W. E., "Isolation and characterization of Staphylococci from human skin II. descriptions of four new species: Staphylococcus warneri, Staphylococcus capitis, Staphylococcus hominis, and Staphylococcus simulans" 25 : 62-79, 1975

14 Schleifer, K. H., "Isolation and characterization of Staphylococci from human skin I. amended descriptions of Staphylococcus epidermidis and Staphylococcus saprophyticus and descriptions of three new species: Staphylococcus cohnii, Staphylococcus haemolyticus, and Staphylococcus xylosus" 25 : 50-61, 1975

15 Bawdon, D., "Identification of axillary Staphylococcus sp. involved in the production of the malodorous thioalcohol 3-methyl-3-sufanylhexan-1-ol" 362 : 2015

16 Ohm, R. A., "Genome sequence of the model mushroom Schizophyllum commune" 28 : 957-, 2010

17 Payne, C. M., "Fungal cellulases" 115 : 1308-1448, 2015

18 Lee, H., "Enhanced removal of PAHs by Peniophora incarnata and ascertainment of its novel ligninolytic enzyme genes" 164 : 10-18, 2015

19 Fey, P. D., "Current concepts in biofilm formation of Staphylococcus epidermidis" 5 : 917-933, 2010

20 Lee, H., "Bioremediation of polycyclic aromatic hydrocarbons in creosote-contaminated soil by Peniophora incarnata KUC8836" 19 : 1-8, 2015