배지에 따른 제브라피쉬(Danio rerio) 배아유래세포의 성장 효과에 관한 연구 = Effect of Culture Media on Embryonic Cell Growth in Zebrafish, Danio rerio

국문 초록 (Abstract)

제브라피쉬 배아 유래세포의 최적 성장조건을 확립하기 위해 3 종류의 배지 DMEM, K-NAC, D-NAC 그룹에서의 세포 성장률을 비교하였다. 실험 결과, 접종밀도에 따른 성장률의 경우, DMEM에 비해 K-NAC 그룹에서 초기 접종 효율이 높게 나타났으며, 후기 성장률은 DMEM 그룹에서 높게 나타났다. K-NAC, DMEM 그룹 모두 FBS 농도에 의한 성장차는 보이지 않았으며, 0.1% embryo extract를 첨가한 배지에서 효과는 낮게 나타났으나 1% trout serum 첨가한 경우 매우 높은 성장률을 보였다(p<0.05). 2-3×105 밀도로 접종한 그룹에서는 유의차가 없었으나 4-5×105 밀도에서는 DMEM 그룹이 K-NAC 그룹 보다 다소 높은 성장률을 보였다(p<0.1). DMEM과 D-NAC 그룹에서의 FBS 농도에 따른 성장률을 비교한 결과, FBS 농도에 따른 성장차는 유의하지 않았으나(p<0.05) D-NAC의 모든 실험군이 DMEM 그룹에 비해 높은 성장률을 보였다(p<0.05).

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