Nucleotide Sequences of a Korean Isolate of Apple Stem Grooving Virus Associated with Black Necrotic Leaf Spot Disease on Pear (Pyrus pyrifolia)

다국어 초록 (Multilingual Abstract)

Pear black necrotic leaf spot (PBNLS) is a disease of
pears caused by capillovirus-like particles, which can
be observed under the electron microscope. The disease
was analyzed by Western blot analysis with antisera
raised against apple stem grooving virus (ASGV)
coat protein. cDNAs covering the entire genome were
synthesized by RT-PCR and RACE using RNA isolated
from Chenopodium quinoa infected with sap extracted
from pear leaves carrying black necrotic spot disease.
The complete genome sequence of the putative pear
virus, 6497 nucleotides in length excluding the poly (A)
tail, was determined and analyzed. It contains two
overlapping open reading frames (ORFs). ORF1, spans
from nucleotide position 37 to 6354, producing a putative
protein of 241 kDa. ORF2, which is in a different
reading frame within ORF1, begins at nucleotide 4788
and terminates at 5750, and produces a putative protein
of 36 kDa. The 241 kDa protein contains sequences
related to the NTP-binding motifs of helicases
and RNA-dependent RNA polymerases. The 36-kDa
protein contains the consensus sequence GDSG found
in the active sites of several cellular and viral serine
proteases. Morphological and serological analysis, and
sequence comparison between the putative pear virus,
ASGV, citrus tatter leaf virus and cherry virus A of the
capillovirus suggest that PBNLS may be caused by a
Korean isolate of ASGV.

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