킬레이트제가 액체배양 중 인삼 부정근의 게르마늄 축적에 미치는 영향

국문 초록 (Abstract)

식물조직배양기술을 이용한 Ge 함유 인삼 부정근 생산 시 Ge의 생산성을 향상시키고자, 다양한 킬레이트제가 인삼 부정근의 Ge 축적과 사포닌 형성 및 생육에 미치는 영향을 조사하였다.
GeO₂ 50ppm과 함께 citric acid, oxalic acid, phosphoric acid, EDTA 및 EGTA와 같은 킬레이트제를 인삼 부정근 배양시 첨가하여 Ge 흡수에 미치는 영향을 조사한 결과, phosphoric acid, EDTA 및 EGTA가 Ge 흡수를 촉진시킨다는 사실을 알 수 있었다. 그러나, EDTA와 EGTA는 인삼 부정근의 생육을 억제시킬 뿐만 아니라 Ge 흡수 촉진 효과도 phosphoric acid에 비해 낮으므로 인삼 부정근의 Ge 흡수를 촉진하는데 가장 효과적인 킬레이트제는 phosphoric acid인 것으로 확인되었다. 인삼 부정근의 Ge 흡수 촉진을 위한 phosphoric acid의 최적 농도는 1.0 mM이었고, 이때의 Ge 함량은22.7±0.3 ㎎%로 대조구(16.8±0.7 ㎎%)의 1.4배에 해당하는 양이다. 한편, 인삼 부정근의 생장률과 총 사포닌 함량은 phosphoric acid의 농도가 증가할수록 감소하는 경향을 나타내었다.

다국어 초록 (Multilingual Abstract)

In order to increase the content of germanium in ginseng adventitious roots, the effects of chelating agents on germanium content and root growth were investigated in the submerged cultures of ginseng adventitious roots. Chelating agents such as citric acid, oxalic acid, phosphoric acid, EDTA (Ethylenediamine tetraacetic acid) or EGTA (Ethylene glycol-bis (β-aminoethylether)-tetraacetic acid) were administrated in the submerged culture of ginseng root containing 50 ppm GeO₂. After 6 weeks of cultivation, fresh weight, germanium and saponin contents in the roots were analyzed. Among chelating agents, addition of 1.0mM phosphoric acid was found to be best for germanium accumulation. Under this condition, germanium content increased 1.4 times as compared to that of the control. The germanium content in the adventitious roots also increased with addition of EDTA or EGTA, while they inhibited the growth of ginseng adventitious root. Citric and oxalic acids were not effective for increasing germanium content in adventitious roots. As the results, it suggests that the phosphoric acid can be proved as the optimal agent for the enhancement of germanium accumulation in ginseng adventitious roots. These results can be served as a guideline for the mass production of ginseng adventitious roots containing germanium by large-scale production.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• 감사의 말씀
• 인용문헌

참고문헌 (Reference)

1 "Tracer study on preferential uptake of germanium by rice plant. Soil Sci. Plant Nutr. 18" 173-179, 1972

2 "Subacute nephrotoxicity of germanium dioxide in the experimental animal. Toxicol. Appl. Pharmacol. 103" 345-353, 1990

3 "Preventive effect of a synthetic immunomodulator on the generation of suppressor macrophages in mice immunized with allogenic lymphocytes. Immunopharmacol. Immunotoxicol. 14" 2 841-846, 1992

4 "Preparation of organic germanium by yeast cell" 23 : 87-90, 1995

5 "Phytoremediation" 49 : 643-668, 1998

6 "Oxygen-nutrition for super health. J. Orthonol. Medicine. 1" 145-150, 1986

7 "Organic germanium stimulate the growth of ginseng adventitious roots and ginsenoside production" 40 : 2959-2961, 2005

8 "Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell culture. Can. J. Bot. 50" 199-2041972

9 "Induction of interferon and activation of NK cells and macrophages in mice by oral administration of Ge-132" 65-74, 1985

10 "Increment of germanium contents in Angelica keiskei Koidz. and Panax Ginseng G.A. Meyer by in vitro propagation" 3 : 251-258, 1995

11 "In vitro culture and increment of germanium content in the genus Agelica L" 1995

12 "Effects of addition of inorganic germanium, GeO2 on the growth, germanium and saponin contents of ginseng adventitious root in submerged culture" 29 : 145-151, 2005

13 "Effect of inorganic germanium in callus growth and organ differentiation of Allium sativum L. J. College of Agriculture Univ. of Wonkwang 13" 21-31, 1990

14 "Effect of germanium treatment in culture medium on germanium absorption by callus induced from brown rice" 41 : 729-735, 1996

15 "Effect of GeO2 and citric acid on germanium content of callus and plant in Angelica koreana MAX" 4 : 101-108, 1996

16 "Dynamics of soil organic acids. Soil Sci." 138-144, 1967

17 "Determination of total saponin in ginseng jellies and candies. Korean J. Food Sci. Technol. 10" 356-360, 1978

18 "Basic studies for increment of germanium contents in Angelica keiskei KOIDZ. and A. acutiloba KITAGAWA" 3 : 45-49, 1995

19 "Accumulation forms of Zn and Pb in Phaseolus vulgaris in the presence and absence of EDTA" 35 : 2854-2859, 2001

20 "A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum. 15" 473-497, 1962