해양균류 Paraboeremia adianticola가 생산하는 Vulculic acid: 벼 도열병 방제를 위한 천연 살균제 = Vulculic acid produced by the marine fungus Paraboeremia adianticola: a natural fungicide for control of Magnaporthe oryzae on rice

다국어 초록 (Multilingual Abstract)

In the course of developing for new antifungal agents to control plant diseases, we discovered that vulculic acid isolated from the culture broth of marine fungus Paraboeremia adianticola SFC20150402-M42 exhibits a promising activity to control rice blast caused by Magnaporthe oryzae. Through in vitro antifungal assay against various plant pathogenic fungi, vulculic acid revealed as a highly selective antifungal compound against M. oryzae. The inhibitory effect of vulculic acid on spore germination and mycelial growth of M. oryzae were investigated using broth microdilution and agar dilution methods. While the spore germination of M. oryzae was completely inhibited by treatment of vulculic acid (6 μg/ml), mycelial growth of M. oryzae was reduced by only 21% at the concentration of 200 μg/ml, compared with that in non-treated controls. The biochemical and molecular biological works on the inhibitory mechanism of vulculic acid in the process of spore germination of M. oryzae are in progress. Taken together, these results indicated that vulculic acid could be used as a selective fungicide for rice blast disease control or serving as a lead compound for new fungicides.

목차 (Table of Contents)

• I. 서론 1
• Ⅱ. 재료 및 방법 6
• 1. 해양곰팡이의 분리 및 선발 6
• 2. 균주 SFC20150402-M24의 동정 7
• 3. 항균활성 물질 분리 8
• 4. 분광학적 분석(spectroscopic analyses) 10
• 5. 7가지 식물병원균에 대한 in vivo 항균활성 검정 11
• 6. 최소억제농도(minimum inhibitory concentration; MIC)시험 14
• 7. 분리한 화합물 1의 벼도열병균 포자발아억제 시험 15
• 8. 분리한 화합물 1의 벼도열병균 균사생장억제 실험 16
• Ⅲ. 결과 및 고찰 17
• 1. 243종 해양곰팡이의 7가지 식물병 방제효과 17
• 2. SFC20150402-M24 균주의 계통학적 분석 19
• 3. 벼 도열병에 대한 항균활성물질의 구조 동정 22
• 4. 식물병원성 진균에 대한 최소억제농도 28
• 5. Vulculic acid의 벼 도열병균 포자발아억제 효과 31
• 6. Vulculic acid의 벼 도열병균 균사생장억제 효과 33
• 7. Vulculic acid의 7가지 식물병 방제효과 35
• Ⅳ. 결론 37
• Ⅴ. 참고문헌 38
• Abstract 44