Biodegradation of C5-C8 Fatty Acids and Production of Aroma Volatiles by Myroides sp. ZB35 Isolated from Activated Sludge

다국어 초록 (Multilingual Abstract)

In the effluents of a biologically treated wastewater from aheavy oil-refining plant, C5-C8 fatty acids including pentanoicacid, hexanoic acid, heptanoic acid, octanoic acid, and2-methylbutanoic acid are often detected. As these residualfatty acids can cause further air and water pollution, a newMyroides isolate ZB35 from activated sludge was explored todegrade these C5-C8 fatty acids in this study. It was foundthat the biodegradation process involved a lag phase thatbecame prolonged with increasing acyl chain length whenthe fatty acids were individually fed to this strain. However,when fed as a mixture, the ones with longer acyl chains werefound to become more quickly assimilated. The branched 2-methylbutanoic acid was always the last one to be depletedamong the five fatty acids under both conditions. Metaboliteanalysis revealed one possible origin of short chain fatty acidsin the biologically treated wastewater. Aroma volatiles including2-methylbutyl isovalerate, isoamyl 2-methylbutanoate,isoamyl isovalerate, and 2-methylbutyl 2-methylbutanoatewere subsequently identified from ZB35 extracts, linking thesource of the fruity odor to these esters excreted by Myroidesspecies. To our best knowledge, this is the first finding of thesearoma esters in bacteria. From a biotechnological viewpoint,this study has revealed the potential of Myroides species asa promising source of aroma esters attractive for food andfragrance industries.

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