Genetic Analysis of the Capsid Region of Norovirus GII.4 Variants Isolated in South Korea

다국어 초록 (Multilingual Abstract)

Norovirus is one of the major causes of non-bacterial gastroenteritisin humans. The aim of this study was to analyzethe amino acid variation of open reading frame 2 of GII.4variants in South Korea during the period from November2006 to December 2012. Sixty-nine complete nucleotide sequencesof open reading frame 2 were obtained from 113GII.4 strains. The GII.4 2006b variants were detected predominantlybetween 2006 and 2009; however, new GII.4variants, which were termed the 2010 variant and the 2012variant, emerged in 2010 and 2012, respectively. The numberof GII.4 2006b variants steadily decreased until 2012,whereas the number of gastroenteritis cases caused by thenew variants increased between 2010 and 2012. The aminoacid sequence in the ORF2 region obtained in this studywas compared with other GII.4 variants isolated in variouscountries. Amino acid variations were observed primarilyat epitope sites and the surrounding regions. Amino acids294, 359, 393, and 413 of the P2 subdomain were the mostvariable sites among the GII.4 variants. The information inthis study can be useful in basic research to predict theemergence and determine the genetic functions of newGII.4 variants.

참고문헌 (Reference)

1 정아용, "인체 노로바이러스의 한국분리주 Hu/NLV/Gunpo/2006/KO의 분자생물학적 특성" 한국미생물학회 45 (45): 105-111, 2009

2 Donaldson, F. E., "Viral shape-shifting: norovirus evasion of the human immune system" 8 : 231-241, 2010

3 Huh, J. W., "Viral etiology and incidence associated with acute gastroenteritis in a 5-year survey in Gyeonggi province, South Korea" 44 : 152-156, 2008

4 Park, K. S., "Updates on the genetic variations of Norovirus in sporadic gastroenteritis in Chungnam Korea, 2009-2010" 9 : 29-, 2012

5 Lam, T. Y. T., "The recombinant origin of emerging human norovirus GII.4/2008: intra-genotypic exchange of the capsid P2 domain" 93 : 817-822, 2012

6 Saitou, N., "The neighbor-joining method: a new method for reconstructing phylogenetic trees" 4 : 406-425, 1987

7 Tan, M., "The P domain of norovirus capsid protein forms a subviral particle that bind to histo-blood group antigen receptor" 79 : 14017-14030, 2005

8 Shanker, S., "Structural analysis of histo-blood antigen biding specificity in a norovirus GII.4 epidemic variant:Implications for epochal evolution" 85 : 8635-8645, 2011

9 Eden, J. S., "Recombination within the pandemic norovirus GII.4 lineage" 87 : 6270-6282, 2013

10 Bill, A. R., "Rapid evolution of pandemic noroviruses of the GII.4 linage" 6 : e1000831-, 2010

11 Vega, E., "Novel surveillance network for norovirus gastroenteritis outbreaks, United States" 17 : 1989-1395, 2011

12 Mathijs, E., "Novel norovirus recombinants and GII.4 sub-lineages associated with outbreaks between 2006 and 2010 in Belgium" 8 : 310-, 2011

13 Siebenga, J. J., "Norovirus illness is a global problem: Emergence and spread of norovirus GII.4 variants, 2001–2007" 200 : 802-812, 2009

14 Zheng, D. P., "Norovirus classification and proposed strain nomenclature" 346 : 312-323, 2006

15 Zakikhany, K., "Molecular evolution of GII-4 norovirus strain" 7 : e41625-, 2012

16 Zheng, D. P., "Molecular epidemiology of genogroup II-genotype 4 norovirus in the United States between 1994 and 2006" 48 : 168-177, 2010

17 Lindesmith, C. L., "Mechanisms of GII.4 norovirus persistence in human population" 5 : 269-290, 2008

18 Bull, A.R., "Mechanisms of GII.4 norovirus evolution" 19 : 233-240, 2011

19 Pang, L. X., "Influence of novel norovirus GII.4 variants on gastroenteritis outbreak dynamics in Alberta and the Northern Territories, Canada between 2000 and 2008" 5 : e11599-, 2008

20 van Beek, J., "Indications for worldwide increased norovirus activity associated with emergence of a new variant of genotype II.4, late 2012" 18 : 8-9, 2013

21 Lindesmith, C. L., "Imminogenetic mechanisms driving norovirus GII.4 antigenic variation" 8 : e1002705-, 2012

22 Motomura, K., "Identification of monomorphic and divergent haplotypes in the 2006-2007 norovirus GII/4 epidemic population by genomewide tracing evolutionary history" 82 : 11247-11262, 2008

23 Ando, T., "Genetic classification of “Norwalk-like Virus”" 181 : S336-S348, 2000

24 Le, V. P., "Genetic characterization of norovirus GII.4 2006b variants from Jeju island, South Korea" 82 : 1665-1070, 2010

25 Park, K. S., "Genetic analysis of norovirus GII.4 variants circulating in Korea in 2008" 155 : 635-641, 2010

26 Park, J. W., "Full sequence analysis and characterization of the South Korean Norovirus GII-4 variant CUK-3" 8 : 167-, 2011

27 Belliot, G., "Evidence of emergence of new GGII.4 norovirus variants from gastroenteritis outbreak survey in France during the 2007-to-2008 and 2008-to-2009 winter season" 48 : 994-998, 2010

28 Martella, V., "Evidence for recomvination berween pandemic GII.4 norovirus strains New Orleans 2009 and Sydney 2012" 51 : 3855-3857, 2013

29 Siebenga, J. J., "Epochal evolution of GGII.4 norovirus capsid proteins from 1995 to 2006" 81 : 9932-9941, 2007

30 Nataraju, S. M., "Emergence of novel norovirus recombinants with NVGII.1/NVGII.5 RdRp gene and NVGII.13 capsid gene among children and adults in Kolkata, India" 2 : 130-137, 2011

31 Han, T. H., "Emergence of norovirus GII-4/2008 variant and recombinant strains in Seoul, Korea" 156 : 323-329, 2011

32 Lindesmith, C. L., "Emergence of a norovirus GII.4 strain correlates with changes in evolving blockade epitopes" 87 : 2803-2813, 2013

33 Haslng, E. M., "Emergence of a new norovirus GII.4 variant and changes in the historical biennial pattern of norovirus outbreak activity in Alberta, Canada, from 2008 to 2013" 51 : 2204-2211, 2013

34 Mahar, J.E., "Characterization of norovirus strains in Australian children from 2006 to 2008: prevalence of recombinant strains" 83 : 2213-2219, 2011