황금(Scutellaria baicalensis) 조직배양에 의한 Baicalin, Baicalein 및 Wogonin 생산 = Production of Baicalin, Baicalein, and Wogonin on Plant Tissue Culture of Scutellaria baicalensis

다국어 초록 (Multilingual Abstract)

Scutellaria baicalensis Georgi (SJ) is a perennial plant and its root has been used in oriental traditional medicine for treatment of fever, inflammation, diarrhea and anticancer effect, etc. In this study, plant tissue culture system for SJ was developed. Stem piece of younger plant was optimum explant for callus induction and growth on MS medium supplemented with NAA 1.0 ㎎/L plus BA 0.5 ㎎/L. Plantlet regeneration through callus culture was well on MS medium containing NAA 1.0 mg/L. SJ has been known biologically active substances such as baicalin, baiclein, and wogonin. This study was carried out to examine the effect of plant growth regulators for production of baicalin, baicalein, and wogonin through callus culture. The HPLC pattern of callus extract was identical to that of standard solution, it shows that the callus produced by tissue culture has the same flavonoids composition of SJ. Baicalin, baicalein, and wogonin production was 471.5~52.8 ㎍/g, 137.6~4.0 ㎍/g, and 16.6~1.3 ㎍/g, respectively, on MS media with nine different plant growth regulator combinations.
This may indicate that plant tissue culture of SJ possible to produce the biologically active substances effectively.

국문 초록 (Abstract)

황금의 식물조직배양 체계 확립 및 2차대사산물 생성 적정조건을 확인하고자 실험을 수행하였다. 황금 조직배양은 MS 배지에 여러 가지 explant를 치상한 결과 어린 식물의 줄기를 사용하여 PGRs인 NAA와 BA를 소량 혼합처리한 조건에서 callus의 분화가 수월하였으며, shoot는 explant에서 발생된 callus를 소량의 NAA가 첨가된 배지에 배양할 때 분화율이 높게 나타났다.
Shoot는 PGRs가 포함되지 않은 MS배지에서 뿌리가 분화되었고 조직배양으로 재분화된 황금 식물개체는 토양으로 이식 후에도 왕성하게 생장함을 확인할 수 있었으며 따라서 본 연구에서는 황금의 식물조직배양 체계를 확립할 수 있었다. 한편, 식물조직배양을 통하여 2차대사 산물 축적이 가능한 지를 확인하고자 황금 callus를 대상으로 몇 가지 flavonids 함량을 HPLC 기기로 분석한 바 callus에서 baicalin, baicalein 및 wogonin을분석할 수 있었다. 이들 함량은 배지에 함유된 PGRs 농도에 따라 baicalin 471.5~52.8 ㎍/g, baicalein 137.6~4.0 ㎍/g, wogonin 16.6~1.3 ㎍/g의 함량 변이를 나타내었다. 따라서 식물조직배양을 통하여 황금에 함유된 baicalin, baicalein 및wogonin 등의 flavonoids를 생산할 수 있음을 확인할 수 있었다.

참고문헌 (Reference)

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