RISS 검색 - 학위논문 상세보기

다국어 초록 (Multilingual Abstract)

Two-dimensional electrophoresis is known as a method capable of efficiently separating proteome expressed in microorganisms, animals and plants by the difference in isoelectric point and molecular weight.
The sum of abundance ratios of all protein spots attempted to be identified was 666.01 (± 73.85). The most expressed protein among the spots was spot No. 451, major allergen Mal d 1 (abundance ratio 54.42), corresponded to 8.2% of the total protein spot expression. The second place of expression amount was spot No. 419 unidentified protein (abundance ratio 28.40), third place 455 MLP-like protein (abundance ratio 24.08), fourth place 480 glutathione S-transterferase (abundance ratio 18.61), fifth place 166 glutamine cytosolic isozyme (abundance) ratio 13.26), 6th place 421 L-ascorbate peroxidase (abundance ratio 12.67), 7th place 163 ß–cyanoalanine synthase (abundance ratio 10.81), 8th place unidentified (abundance ratio 10.16), 9th place 452 major allergen Mal d 1 (abundance ratio 8.80) and 165 glutamine cytosolic isozyme (abundance ratio 8.45). Since three of the top ten proteins expressed in the peel were allergic, it was thought that the apple peel contained a large amount of allergic protein.
As a result of 2-DE with proteins extracted from apple peel and flesh, 30 proteins were consistently and repeatedly differing in expression levels more than 2 times in the peel and flesh. Coring the 30 protein spots and analyzing them by MALDI-TOF / TOF, 23 proteins were identified, but 7 spots of 4, 36, 211, 212, 388, 470 and 495 were not identified.
Among the identified proteins, spot 8 chaperonin 60 subunit alpha 1, chloroplastic and spot 380 NADP-dependent D-sorbitol-6-phosphate dehydrogenase showed 2.1 and 3.1 times higher expression levels in the flesh than those of the peel.
Compared to the flesh, the skin peels had a spot No. 21 1-aminocyclopropane-1-carboxylate oxidase homolog 6.5 times, No. 148 acetyl-CoA acetyltransferase, cytosolic 1 4.6 times, 210 oxygen-evolving enhancer protein 1, chloroplastic 19.3 times, 216 times And 219 (+)-neomenthol dehydrogenase-like are 4.2 and 71.0 times, 217 hypothetical protein OsI_18159 is 10.2 times, 221 L-ascorbate peroxidase 2 is 17.5 times, ribulose-1,5-bisphosphate carboxylase / oxygenase 273, respectively. Large subunit 10.1 times, 305 formate dehydrogenase, mitochondrial 6.1 times, 316 acetyl-CoA acetyltransferase, cytosolic 1-like 8.1 times, 348 8-hydroxygeraniol dehydrogenase-like 13.8 times, 391 and 392 acidic endochitinase SE2-like and partial were 2.6 and 3.1 times respectively, MLP-like protein 34 was 8.0 times at 406, major allergen Mal d 1 at 451 and 452 was 5.6 and 18.0 times at 451 and 97.3 at major allergen Mal d 1.06A01, respectively. Pear 472 is an uncharacterized protein that is believed to respond to the plant hormone ABA. At5g02240-like expressed 39.0 fold, 483 and 484 glutathione S-transferase-like 5.9 and 5.6 fold, and 489 uncharacterized protein LOC103408523 66.8 fold higher.
The expression levels of spot No. 21 1-aminocyclopropane-1-carboxylate oxidase homolog, No. 148 acetyl-CoA acetyltransferase, cytosolic 1, No. 210 oxygen-evolving enhancer protein 1, chloroplastic, No. 216 and 219 (+)-neomenthol dehydrogenase-like, No. 221 L-ascorbate peroxidase 2, No. 273 ribulose-1,5-bisphosphate carboxylase / oxygenase large subunit, No. 305 formate dehydrogenase, mitochondrial, No. 316 acetyl-CoA acetyltransferase, cytosolic 1-like, No. 348 8-hydroxygeraniol dehydrogenase-like, No. 391 and 392 acidic endochitinase SE2-like, partial, No. 406 MLP-like protein 34, No. 451 and 452 major allergen Mal d 1, No. 472 uncharacterized protein At5g02240-like (presumed to respond to plant hormone ABA), No. 483 and 484 glutathione S-transferase-like and No. 489 uncharacterized protein LOC103408523 were 6.5 and 4.6, 19.3, 4.2, 71.0, 10.2, 17.5, 10.1, 6.1, 8.1, 13.8, 2.6, 3.1, 8.0, 5.6, 18.0, 97.3, 39.0, 5.9, 5.6 and 66.8 times higher in the peel than in the flesh, respectively.
On the other hand, the peel is more exposed to physical, chemical and biologically disadvantageous environment than the flesh. Therefore, secondary metabolite metabolism (spots 148, 316, 348), defense and stress response-related proteins (spots 216, 219, 305, 483, and 484), chitinase involved in chitin metabolism spots 391 and 392) and proteins with allergenic properties (spots 406, 451, 452 and 457), which are more expressed in the peel than flesh, will provide the impetus for maintaining and exerting properties including fruit growth and maturation. In particular, the apple peel has a significantly higher expression level of allergic proteins than the flesh, so those who are sensitive to apple allergy need to be very careful when ingesting it.

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