콩 검은뿌리썩음병균(Calonectria ilicicola)의 형태적 특징과 유전자 수준에서의 동정

다국어 초록 (Multilingual Abstract)

The 18 isolates of soybean red crown rot pathogen (Calonectria ilicicola) were isolated from infected soybean
plants showing chlorosis symptom and red perithecia sign in 2002 and 2003 season in Korea. The morphological
characteristics of the typical isolate SC03-15, such as macroconidia and macrovesicles in anamorph as
well as perithecia, asci and ascospores in teleomorph, were observed from red crown rot pathogens. The perithecia
were red-brown globose to ovoid, and 357.9 (312.5~391.5.7) μm high and 474.3 (419~535) μm diameter.
Asci were hyaline and clavate and included 8 ascospores, which were straight to slightly curved, 1(-3)-septate,
and 36.9(31.1~49.4) × 5.2(4.5~6.1) μm. Macroconidia in anamorph were cylindrical, hyaline and in size 73.2
(68.1~77.6) × 5.8(4.9~6.8) μm. Further identification process was performed through molecular method, such
as a phylogenetic analysis by rDNA sequencing of ITS and β-tubuline genes of all isolates tested in this study.
All isolates were identified as Calonectria ilicicola by DNA sequencing of ITS and β-tubuline genes, and morphological
characteristics.

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