Effects of 4-Nonlyphenol Exposure on P. olivaceus and S. schlegeli Vitellogenesis

다국어 초록 (Multilingual Abstract)

The effects of the estrogenic compound 4-nonylphenol (4-NP) on vitellogenesis in juvenile olive flounder (Paralichthys olivaceus) and rockfish (Sebastes schlegeli) exposed continuously at 10, 50 and 100 ㎍ ℓ^(－1) levels for 7 days were compared. The expression of VTG mRNA level and protein using specific probes were examined. The levels of plasma estradiol-17β (E_2) and testosterone (T) were assessed by radioimmunoassay (RIA). Plasma E2 concentrations increased significantly in two female fish species exposed to 100 ㎍ ℓ^(－1) of 4-NP over concentrations in control fish. Plasma T concentrations increased in P. olivaceus. Four days after exposure, the level of VTG mRNA expression increased in P. olivaceus and S. schlegeli exposed to 20 ㎍ ℓ^(－1) of 4-NP. In addition, plasma VTG protein expression was seen in P. olivaceus and S. schlegeli. In S. schlegeli and P. olivaceus exposed to 4-NP, the changes were noticed mainly in hepatocytic vaculation after 7 days of exposure. Thus, 4-NP may disrupt vitellogenesis in immature fish both directly and indirectly via disrupted steroidogenesis and liver pathology. Immature S. schlegeli were the most sensitive to 4-NP exposure in vitellogenesis.

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