Inhibitory Effects of Honokiol and Magnolol on Biofilm Formation by Acinetobacter baumannii

다국어 초록 (Multilingual Abstract)

Acinetobacter baumannii is a nosocomial pathogen that can survive unfavorable conditions, such as, desiccation, nutrient starvation, and antimicrobial treatment, and this is primarily due to its ability to form biofilms on biotic and abiotic surfaces like tissues and medical devices.
In this study, honokiol and magnolol were investigated for antibiofilm activity against A. baumannii ATCC 17978. Both were found to inhibit biofilm formation dose-dependently and to disperse matured biofilms. Honokiol and magnolol were found to inhibit biofilm formation by five and four of eight additional clinical A. baumannii isolates, respectively.
Furthermore, honokiol and magnolol effectively suppressed pellicle formation and the surface motilities of the A.
baumannii and prolonged the survival of infected nematode Caenorhabditis elegans. These results demonstrate that honokiol and magnolol may be useful for controlling A. baumannii infections.

참고문헌 (Reference)

1 이도현, "Use of Nanoscale Materials for the Effective Prevention and Extermination of Bacterial Biofilms" 한국생물공학회 23 (23): 1-10, 2018

2 Choi, A. H, "The pgaABCD locus of Acinetobacter baumannii encodes the production of poly-β-1-6-Nacetylglucosamine, which is critical for biofilm formation" 191 : 5953-5963, 2009

3 Mussi, M. A, "The opportunistic human pathogen Acinetobacter baumannii senses and responds to light" 192 : 6336-6345, 2010

4 Young-Soo Kim, "Synthesis and Microbiological Evaluation of Honokiol Derivatives as New Antimicrobial Agents" 대한약학회 33 (33): 61-65, 2010

5 Golic, A, "Staring at the cold sun : blue light regulation is distributed within the genus Acinetobacter" 8 : e55059-, 2013

6 McBride, M. J, "Shining a light on an opportunistic pathogen" 192 : 6325-6326, 2010

7 Yang, M, "Role of oxidative stress in the induction of metallothionein-2A and heme oxygenase-1 gene expression by the antineoplastic agent gallium nitrate in human lymphoma cells" 45 : 763-772, 2008

8 Cruz-Muniz, M. Y, "Repurposing the anticancer drug mitomycin C for the treatment of persistent Acinetobacter baumannii infections" 49 : 88-92, 2017

9 Karki, R, "Magnoliae Cortex inhibits intimal thickening of carotid artery through modulation of proliferation and migration of vascular smooth muscle cells" 50 : 634-640, 2012

10 Rajasekharan, S. K, "LED based real-time survival bioassays for nematode research" 8 : 11531-, 2018

11 Mikkelsen, H, "Key twocomponent regulatory systems that control biofilm formation in Pseudomonas aeruginosa" 13 : 1666-1681, 2011

12 Jayamani, E, "Insect-derived cecropins display activity against Acinetobacter baumannii in a whole-animal high-throughput Caenorhabditis elegans model" 59 : 1728-1737, 2015

13 Zhou, P, "In vitro inhibitory activities of magnolol against Candida spp" 11 : 2653-2661, 2017

14 Park, J, "In vitro antibacterial and anti-inflammatory effects of honokiol and magnolol against Propionibacterium sp" 496 : 189-195, 2004

15 Kim, H. I, "In vitro and in vivo antimicrobial efficacy of natural plant-derived compounds against Vibrio cholerae of O1 El Tor Inaba serotype" 79 : 475-483, 2015

16 Giles, S. K, "Identification of genes essential for pellicle formation in Acinetobacter baumannii" 15 : 116-, 2015

17 Iwashkiw, J. A, "Identification of a general O-linked protein glycosylation system in Acinetobacter baumannii and its role in virulence and biofilm formation" 8 : e1002758-, 2012

18 Solinski, A. E, "Honokiol-inspired analogs as inhibitors of oral bacteria" 4 : 118-122, 2018

19 Sun, L, "Honokiol induces reactive oxygen speciesmediated apoptosis in Candida albicans through mitochondrial dysfunction" 12 : e0172228-, 2017

20 Marti, S, "Growth of Acinetobacter baumannii in pellicle enhanced the expression of potential virulence factors" 6 : e26030-, 2011

21 Lee, J. H, "Ginkgolic acids and Ginkgo biloba extract inhibit Escherichia coli O157 : H7 and Staphylococcus aureus biofilm formation" 174 : 47-55, 2014

22 Clemmer, K.M, "Genetic analysis of surface motility in Acinetobacter baumannii" 157 : 2534-2544, 2011

23 Chitambar, C. R., "Gallium and its competing roles with iron in biological systems" 1863 : 2044-2053, 2016

24 Lee, J. H, "Flavone reduces the production of virulence factors, staphyloxanthin and α-hemolysin, in Staphylococcus aureus" 65 : 726-732, 2012

25 Luo, L. M, "Enhancing pili assembly and biofilm formation in Acinetobacter baumannii ATCC 19606 using nonnative acyl-homoserine lactones" 15 : 62-, 2015

26 Sun, L, "Effects of magnolol and honokiol on adhesion, yeast-hyphal transition, and formation of biofilm by Candida albicans" 10 : e0117695-, 2015

27 Espinal, P, "Effect of biofilm formation on the survival of Acinetobacter baumannii on dry surfaces" 80 : 56-60, 2012

28 Nait Chabane, Y, "Characterisation of pellicles formed by Acinetobacter baumannii at the air-liquid interface" 9 : e111660-, 2014

29 Beceiro, A, "Biological cost of different mechanisms of colistin resistance and their impact on virulence in Acinetobacter baumannii" 58 : 518-526, 2014

30 Babapour, E, "Biofilm formation in clinical isolates of nosocomial Acinetobacter baumannii and its relationship with multidrug resistance" 6 : 528-533, 2016

31 Pour, N. K, "Biofilm formation by Acinetobacter baumannii strains isolated from urinary tract infection and urinary catheters" 62 : 328-338, 2011

32 Kaiser, D, "Bacterial swarming : a re-examination of cellmovement patterns" 17 : R561-R570, 2007

33 Tomaras, A. P, "Attachment to and biofilm formation on abiotic surfaces by Acinetobacter baumannii: involvement of a novel chaperone-usher pili assembly system" 149 : 3473-3484, 2003

34 Syu, W. J, "Antimicrobial and cytotoxic activities of neolignans from Magnolia officinalis" 1 : 530-537, 2004

35 Satish Kumar Rajasekharan, "Antibiofilm and Anti-β-Lactamase Activities of Burdock Root Extract and Chlorogenic Acid against Klebsiella pneumoniae" 한국미생물·생명공학회 27 (27): 542-551, 2017

36 Sakaue, Y, "Anti-biofilm and bactericidal effects of magnolia bark-derived magnolol and honokiol on Streptococcus mutans" 60 : 10-16, 2016

37 Dijkshoorn, L, "An increasing threat in hospitals : multidrug-resistant Acinetobacter baumannii" 5 : 939-951, 2007

38 Manoharan, R. K, "Alizarin and chrysazin inhibit biofilm and hyphal formation by Candida albicans" 7 : 447-, 2017

39 Eijkelkamp, B. A, "Adherence and motility characteristics of clinical Acinetobacter baumannii isolates" 323 : 44-51, 2011

40 Bergogne-Berezin, E, "Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features" 9 : 148-165, 1996

41 Howard, A, "Acinetobacter baumannii: an emerging opportunistic pathogen" 3 : 243-250, 2012

42 Harding, C. M, "Acinetobacter baumannii strain M2produces type IV pili which play a role in natural transformation and twitching motility but not surface-associated motility" 4 : 2013

43 Runci, F, "Acinetobacter baumannii biofilm formation in human serum and disruption by gallium" 61 : 2017

44 Antunes, L. C, "Acinetobacter baumannii : evolution of a global pathogen" 71 : 292-301, 2014

45 Chen, R, "A1S_2811, a CheA/Y-like hybrid twocomponent regulator from Acinetobacter baumannii ATCC17978, is involved in surface motility and biofilm formation in this bacterium" 6 : 2017

46 Murphy, C. T, "(2003)Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans" 424 : 277-283, 2003