Physiological effects of copper on the freshwater alga Closterium ehrenbergii Meneghini (Conjugatophyceae) and its potential use in toxicity assessments

다국어 초록 (Multilingual Abstract)

Although green algae of the genus Closterium are considered ideal models for testing toxicity in aquatic ecosystems,little data about the effects of toxicity on these algal species is currently available. Here, Closterium ehrenbergii was usedto assess the acute toxicity of copper (Cu). The median effective concentration (EC50) of copper sulfate based on a dose responsecurve was 0.202 mg L-1, and reductions in photosynthetic efficiency (Fv / Fm ratio) of cells were observed in culturesexposed to Cu for 6 h, with efficiency significantly reduced after 48 h (p < 0.01). In addition, production of reactive oxygenspecies significantly increased over time (p < 0.01), leading to damage to intracellular organelles. Our results indicate thatCu induces oxidative stress in cellular metabolic processes and causes severe physiological damage within C. ehrenbergiicells, and even cell death; moreover, they clearly suggest that C. ehrenbergii represents a potentially powerful test modelfor use in aquatic toxicity assessments.

참고문헌 (Reference)

1 Juneau, P, "Use of chlorophyll fluorescence of Closterium ehrenbergii and Lemna gibba for toxic effect evaluation of sewage treatment plant effluent and its hydrophobic components" 55 : 1-8, 2003

2 Guo, R, "Transcriptomic profiles reveal the genome-wide responses of the harmful dinoflagellate Cochlodinium polykrikoides when exposed to the algicide copper sulfate" 17 : 29-, 2016

3 Kim, S. -G, "Toxicity test of anionic and nonionic surfactants to Closterium ehrenbergii by new indexes" 27 : 274-281, 1998

4 Li, X, "Toxicity of cypermethrin on growth, pigments, and superoxide dismutase of Scenedesmus obliquus" 60 : 188-192, 2005

5 Viana, S. M, "The toxicity of copper sulphate and atrazine to the diatom Aulacoseira Granulata (Ehrenberg)Simmons" 17 : 291-300, 2005

6 Knauert, S, "The role of reactive oxygen species in copper toxicity to two freshwater green algae" 44 : 311-319, 2008

7 Mamboya, F. A, "The effect of copper on the daily growth rate and photosynthetic efficiency of the brown macroalga Padina boergesenii" 185-192, 1999

8 Trampe, E, "Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging" 158 : 1667-1675, 2011

9 Vinitha Ebenezer, "Quantification of the Sub-lethal Toxicity of Metals and Endocrine-disrupting Chemicals to the Marine Green Microalga Tetraselmis suecica" 한국수산과학회 16 (16): 187-194, 2013

10 Ichimura, T, "Post-zygotic isolation between allopatric mating groups of Closterium ehrenbergii Meneghini (Conjugatophyceae)" 23 : 77-85, 1984

11 Guo, R, "PmMGST3, a novel microsomal glutathione S-transferase gene in the dinoflagellate Prorocentrum minimum, is a potential biomarker of oxidative stress" 546 : 378-385, 2014

12 Sathasivam, R, "Physiological and biochemical responses of the freshwater green algae Closterium ehrenbergii to the common disinfectant chlorine" 133 : 501-508, 2016

13 Giardi, M. T, "Photosystem II-based biosensors for the detection of pollutants" 16 : 1027-1033, 2001

14 Sabatini, S. E, "Oxidative stress and antioxidant defences in two green microalgae exposed to copper" 72 : 1200-1206, 2009

15 OECD, "OECD guidelines for the testing of chemicals. Test No. 201. Freshwater algal and cyanobacteria, growth inhibition test" OECD Publications 25-, 2006

16 Watanabe, M. M, "NIES collection list of strains. 6th ed. 2000 microalgae and protozoa. Microbial Culture Collections"

17 Ishikawa, T, "Hydrogen peroxide generation in organelles of Euglena gracilis" 33 : 1297-1299, 1993

18 Guo, R, "Genome-wide analysis of transcription and photosynthesis inhibition in the harmful dinoflagellate Prorocentrum minimum in response to the biocide copper sulfate" 57 : 27-38, 2016

19 Lewis, M. A, "Fundamentals of Aquatic Toxicology: Effects, Environmental Fate, and Risk Assessment" Taylor and Francis 135-169, 1995

20 Chen, H, "Evaluation of the role of the glutathione redox cycle in Cu (II) toxicity to green algae by a chiral perturbation approach" 120-121 : 19-26, 2012

21 Lee, M. -A, "Evaluation and selection of reference genes for ecotoxicogenomic study of the green alga Closterium ehrenbergii using quantitative real-time PCR" 24 : 863-872, 2015

22 U.S. Environmental Protection Agency, "Environmental standards. General standards: General standards for discharge of environmental pollutant"

23 Ebenezer, V, "Effects of the algicides CuSO4 and NaOCl on various physiological parameters in the harmful dinoflagellate Cochlodinium polykrikoides" 26 : 2357-2365, 2014

24 Cairns, J, "Effects of temperature on aquatic organism sensitivity to selected chemicals" Virginia Water Resources Research Center 88-, 1978

25 Qian, H. F, "Effects of glufosinate on antioxidant enzymes, subcellular structure, and gene expression in the unicellular green alga Chlorella vulgaris" 88 : 301-307, 2008

26 Magdaleno, A, "Effects of cadmium, copper and zinc on growth of four isolated algae from a highly polluted Argentina river" 92 : 202-207, 2013

27 Franklin, N. M, "Effect of initial cell density on the bioavailability and toxicity of copper in microalgal bioassays" 21 : 742-751, 2002

28 Manimaran, K, "Effect of copper on growth and enzyme activities of marine diatom, Odontella mobiliensis" 88 : 30-37, 2012

29 Young, R. G, "Effect of copper and silver ions on algae" 44 : 1643-1647, 1972

30 Qin, Y, "Dihydrorhodamine 123 is superior to 2,7-dichlorodihydrofluorescein diacetate and dihydrorhodamine 6G in detecting intracellular hydrogen peroxide in tumor cells" 32 : 224-228, 2008

31 Lee, M. -A, "Different transcriptional responses of heat shock protein 20 in the marine diatom Ditylum brightwellii exposed to metals and endocrine-disrupting chemicals" 29 : 1379-1389, 2014

32 Fukumoto, R. -H, "Detection and evaluation of a novel sexual pheromone that induces sexual cell division of Closterium ehrenbergii (Chlorophyta)" 33 : 441-445, 1997

33 Ferrat, L, "Assessment of the use of biomarkers in aquatic plants for the evaluation of environmental quality: application to seagrasses" 65 : 187-204, 2003

34 Schreiber, U, "Assessment of photosystem II photochemical quantum yield by chlorophyll fluorescence quenching analysis" 22 : 209-220, 1995

35 Kim, J. -D, "Alga-lytic activity of Pseudomonas fluorescens against the red tide causing marine alga Heterosigma akashiwo (Raphidophyceae)" 41 : 296-303, 2007

36 Antón, F. A, "Acute toxicity of technical captan to algae and fish" 50 : 392-399, 1993

37 Sbihi, K., "Accumulation and toxicological effects of cadmium, copper and zinc on the growth and photosynthesis of the freshwater diatom Planothidium lanceolatum(Brébisson)Lange-Bertalot : A laboratory study" 3 (3): 497-506, 2012

38 Sato, M, "A simple and rapid dual-fluorescence viability assay for microalgae" 20 : 53-59, 2004