미역취(Solidago virga - aurea var. gigantea Miq.)뿌리 추출물이 MC3T3 - E1 조골세포의 활성과 분화에 미치는 영향

국문 초록 (Abstract)

골조직은 골아세포, 파골세포, 골세포 등으로 구성되며, 골개조시 여러 인자가 세포증식, 분화, 활성화 및 골대사 조절에 관여한다. 이때 조골세포의 활성은 골형성에 중요하므로, 본 연구에서는 MC3T3-E1 조골세포주를 이용하여 식용 자원인 미역취뿌리의 조골세포의 증식과 분화활성에 미치는 영향을 조사하였다. 미역취뿌리 메탄올 추출물 및 분획물이 조골세포의 성장에 미치는 영향을 MTT 검색법으로 조사한 결과, 미역취뿌리 메탄올 추출물 10, 100 ㎍/mL 처리시 대조군과 비교하여 각각 140, 120% 증가하여 조골세포의 높은 성장률을 보였다. 미역취 뿌리 추출물 및 순차 분획물 시료가 ALP 효소 활성에 미치는 영향을 3일 간격으로 배지 교환 및 시료처리를 하면서 27일 동안 배양시간에 따른 변화를 측정하여 조사하였다. 그 결과, 농도 1, 10, 100 ㎍/mL 처리시 n-hexane과 chloroform 분획을 제외한 나머지 분획물들이 시간이 지남에 따라 약간의 ALP 활성을 증가시켰고, 메탄올 추출물은 27일째에 대조군에 비해 약 4.4배 이상, 양성대조군에 비해 약 2배 이상 ALP 활성을 증가시켰다. 미역취뿌리 메탄올추출물은 다시 ALP 효소 염색법과 alizarin red 염색으로 조골세포의 ALP활성유도, 분화와 석회화 형성능을 재확인하였다. 따라서 미역취뿌리 추출물중 골세포의 활성을 촉진시키는 물질은 극성에 따른 분획물보다 methanol 추출물에서 활성이 높게 나타나는 결과로 미뤄볼 때, 단일성분에 의한 것보다 복합적 성분들의 상승 작용에 의하여 조골세포의 증식과 분화를 증진시킬 수 있다고 할 수 있다. 아울러 종래의 골질환에 좋다고 알려진 식품과 양성대조군에 비해 빠르게 유도하고 있어 앞으로 미역취뿌리에 대한 분자생물학 수준 등의 구체적인 연구들과 기작연구가 지속적으로 이루어져야 할 것이다.

다국어 초록 (Multilingual Abstract)

The purpose of this study was to examine the ability of alkaline phosphatase (ALP) synthesis of MC3T3-E1 cells when above edible sources, Solidago virga-aurea var. gigantea Miq. root (SVR) extracts, were supplimented. MC3T3-E1 cells were cultured with α-MEM (vehicle control), dexamethasone and genestein (positive control), and SVR extracts for 27 days. The effects of SVR MeOH extracts and its fractions on cell proliferation were measured by MTT assay. At 10, 100 ㎍/mL of SVR methanol extract treated, that were elevated of cell proliferation to 140 and 120% via vehicle control, respectively. And then ALP synthesis was measured by spectrophotometer for enzyme activity and by naphthol AS-BI staining for morphometry at 3, 9, 18, and 27th day. As the results, every extracts and fractions were promoted ALP activity by time course at 1, 10, 100㎍/mL, except n-hexane and chloroform fractions. Remarkably, the MeOH extracts were increased ALP activity more than 4.4 times compared with vehicle control, 2.2 times via positive control at 27th day (p＜0.05). The SVR MeOH extracts treated cells, especially at a concentration of 10 ㎍/mL, showed remarkably higher than vehicle-treated control cells of mineralization which were checked by Alizarin red staining. These results indicate that SVR methanol extract have an induction ability of proliferation and differentiation on osteoblast.

목차 (Table of Contents)

• Abstract
  
• 서론
  
• 재료 및 방법
  
• 결과 및 고찰
  
• 요약
  
• 감사의 글
  
• 문헌

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