Determination of Eperisone in Human Plasma by Liquid Chromatography-ESI-Tandem Mass Spectrometry

다국어 초록 (Multilingual Abstract)

A sensitive and selective method for the determination of 4’-ethyl-3-methyl-3-piperidinopropiophenone
hydrochloride (eperisone hydrochloride) in human plasma was developed and validated.
The procedure employed an internal standard and a solvent extraction step followed by
chromatography on a Xterra C18 minibore column. Detection was by electrospray ionization
tandem mass spectrometry with multiple reaction monitoring. The mass transitions of
eperisone and tolperisone (IS) were m/z 260 → 98 and m/z 246 → 98, respectively. The
method has a limit of detection of 0.1 pg/mL for eperisone based on the three times signal-tonoise
value with a linear range from 0.01 to 10.0 ng/mL for the analyte. Extraction recovery
was on average 98.6±7.2% (SD) for eperisone. The Intra- and inter-day assay accuracy
ranged from 93 to 114% and precision (RSD) was better than 8.5%. The method was successfully
employed to analyze plasma samples and evaluate pharmacokinetics of eperisone in
healthy male volunteers.

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