선천성 거대결장증 진단에 필요한 조직화학염색 : Rapid Acetylcholinesterase Staining = Histochemical Stain in the Diagnosis of Hirschsprung's Disease

다국어 초록 (Multilingual Abstract)

Hirschsprung's disease is a congenital absence of ganglion cells in the Meissner's and Auerbach's plexuses of the colon. The definitive diagnosis of the disease can only be based on histologic examination of bowel wall biopsies demonstrating aganglionosis. To distinguish Hirschsprung's disease from other causes of contipation and megacolon, it is helpful to use histologic examination with routine hematoxylin and eosin (H&E) staining and acetylcholinesterase (AChE) special histochemical staining techniques. With H&E staining, the difficulties may arise in the hypoganglionic area of the transition zone, or in hypoganglionosis or neuronal intestinal dyspiasia. The AChE technique provides reliable identification of ganglion cells by staining nerve filaments in close proximity to the ganglion cells. However, the conventional AChE technique takes about 2 hours for the examination of specimens, so it is not suitable for intraoperative examination. Using a rapid AChE histochemical staining technique, it is possible to produce staining of cholinergic fibers in 20 minutes. It should enable the surgeon to use it selectively during operation and to support or refute the H&E findings.