Identification and Phylogenetic Relationship at Cytochrome Oxidase Subunit I (COI) Gene among Korean Terrestrial Planarian Taxa

국문 초록 (Abstract)

미토콘드리아 산화효소(COI) 유전자의 서열을 이용하여 한국 내 육지플라나리아의 분류와 계통관계를 규명하였다. 유전자은행에서 Bipaliidae과의 종에 관한 기 발표된 서열을 계통분석을 위해 포함시켰다. 육지 플라나리아의 서열 배당은 387 bp에서 444 bp로 나타났으며 이런 차이는 염기 삽입에 기인하였다. COI 분석에 근거한 계통학적 분지도는 형태적 형질에 의한 결과와 일치하지 않았다. Bipalium nobile가 나머지 분류군(Bipalium adventitium, Bipalium venosum, Bipalium kewense, Bipalium multilineatum)을 포함하는 관계로 나타났다. 내부 가지의 분지군은 강하게 지지되었다(＞91%). The phylogenic tree on COI 분석에 의한 계통도는 잘 분리되었다. 이들은 단계원을 형성하였다. 미토콘드리아 산화효소 유전자는 한국 내 육지 플라나리아 분류군을 동정하는데 유력한 도구가 될 수 있다.

다국어 초록 (Multilingual Abstract)

Sequence data of Cytochrome Oxidase Subunit I (COI) gene of mitochondria were used to elucidate the taxonomy and phylogenetic relationships of the terrestrial planarian taxa in Korea. Published COI gene sequences from Family Bipaliidae in GenBank were also included in the phylogenetic analysis. The aligned data sets for Terricola ranged from 387 to 444 nucleotides (bp) as a result of differences in insert nucleotides. The phylogeny based on COI analysis was not congruenced with the morphological traits. Bipalium nobile included the remainder taxa (Bipalium adventitium, Bipalium venosum, Bipalium kewense, and Bipalium multilineatum). Internal nodes were strongly supported (＞91%). The phylogenetic tree on COI analysis showed that most identified species were well separated from each other. The main phylogenetic analysis formed monophyletic groups. COI gene of mitochondria could have the resolving power for taxonomy information for the terrestrial planarian taxa in Korea.

목차 (Table of Contents)

• Introduction
• Materials and Methods
• Results
• Discussion
• References
• 초록

참고문헌 (Reference)

1 Kawakatsu, M., "corrections of the previous land planarian indices of the world (Turbellaria, Seriata, Tricladida, Terricola). Additions and corrections of the previous land planarian indices of the world-10" 40 : 157-177, 2002

2 Steinbock,O, "Zur Systematik der Turbellaria metamerata, zugleich ein Beitrag zur Morphologie des Tricladen-Nervensystems" 64 : 165-192, 1925

3 Ball,I.R, "The phyletic status of the Paludicola" 84 : 7-12, 1981

4 Baguna, J, "The nervous system in planarians: peripheral and gastrodermal plexuses, pharynx innervation, and the relationship between central nervous system structure and the acoelomate organization" 155 : 237-252, 1978

5 Saitou, N, "The neighbor-joining method: A new method for reconstructing phylogenetic trees" 4 : 406-425, 1987

6 Mateos, E., "Terrestrial planarians (Platyhelminthes, Tricladida, Terricola) from the Iberian Peninsula: first records of the family Rhynchodemidae, with the description of a new Microplana species" 67 : 267-276, 1998

7 Salo, E, "Regeneration in planarians and other worms: new findings, new tools, and new perspectives" 292 : 528-539, 2002

8 Bessho, Y., "Planarian mitochondria I. Heterogeneity of cytochrome c oxidase subunit I gene sequences in the freshwater planarian, Dugesia japonica" 34 : 324-330, 1992

9 Sluys,R, "Phylogenetic relationships of the triclads (Platyhelminthes, Seriata, Tricladida)" 59 : 3-25, 1989

10 Felsenstein, J, "PHYLIP (Phylogeny Inference Package) version 3.5s. Distributed by the Author" Department of Genetics, Univ. Washington Press 1993

11 Kawakatsu, M., "On the places of origin of three, very large Bipaliid land planarians from Japan (Turbellaria, Seriata, Tricladida, Terricola)" 22 : 39-52, 2001

12 Ball, I. R, "On the phylogenetic classification of aquatic planarians" 154 : 21-35, 1977

13 Alvarez-Presas, M., "Molecular phylogeny of land and freshwater planarians (Tricladida, Platyhelminthes): From freshwater to land and back" 47 : 555-568, 2008

14 Tamura, K., "MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0" 24 : 1596-1599, 2007

15 Winsor, L., "Introduction, and ecological and systematic background, to the Terricola (Tricladida)" 42 : 389-404, 1998

16 Sluys,R, "Global diversity of land planarians (Platyhelminthes, Tricaldida, Terricola): a new indicator-taxon in biodiversity and conservation studies" 8 : 1663-1681, 1999

17 Kawakatsu, M., "Distribution records of Bipalium nobile in Japan (Turbellarea, Tricladida, Seriata, Terricola)" 19 : 25-32, 1998

18 Ogren, R. E., "Additions and corrections of the previous land planarian indices of the world (Turbellaria, Seriata, Tricladida, Terricola). Addenuum Ⅳ. Geographic locus index: Bipalidae; Rhynchodemidae (Rhychodeminae; Microplaninae); Geopanidae (Geoplaninae; Caenoplaninae; Pelmatoplaninae)" 35 : 63-103, 1997

19 Carranza, S., "A robust molecular phylogeny of the Tricladida (Platyhelminthes: Seriata) with a discussion on morphological synapomorphies" 265 : 631-640, 1998

20 Carranza, S., "A reappraisal of the phylogenetic and taxonomic position of land planarians (Platyhelminthes, Thrbellaria, Tricladida) inferred from 18S rDNA sequences" 42 : 433-440, 1998

21 Froehlich,C.G, "A contribution to the zoogeography of Neotropical land planarians" 23 : 153-162, 1967