DNA Barcoding of Fish, Insects, and Shellfish in Korea

다국어 초록 (Multilingual Abstract)

DNA barcoding has been widely used in species identification and biodiversity research. A short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) sequence serves as a DNA bio-barcode. We collected DNA barcodes, based on COI sequences from 156 species (529 sequences) of fish, insects, and shellfish. We present results on phylogenetic relationships to assess biodiversity the in the Korean peninsula. Average GC% contents of the 68 fish species (46.9%), the 59shellfish species (38.0%), and the 29 insect species (33.2%) are reported. Using the Kimura 2 parameter in all possible pairwise comparisons, the average interspecific distances were compared with the average intraspecific distances in fish (3.22 vs. 0.41), insects (2.06 vs. 0.25), and shellfish (3.58 vs. 0.14). Our results confirm that distance-based DNA barcoding provides sufficient information to identify and delineate fish, insect, and shellfish species by means of all possible pairwise comparisons. These results also confirm that the development of an effective molecular barcode identification system is possible. All DNA barcode sequences collected from our study will be useful for the interpretation of species-level identification and community-level patterns in fish, insects, and shellfish in Korea, although at the species level, the rate of correct identification in a diversified environment might be low.

참고문헌 (Reference)

1 Singer GA, "iBarcode.org: web-based molecular biodiversity analysis" 10 (10): 14-, 2009

2 Du H, "The correlation coefficient of GC content of the genome-wide genes is positively correlated with animal evolutionary relationships" 584 : 3990-3994, 2010

3 Ward RD, "The campaign to DNA barcode all fishes, FISH-BOL" 74 : 329-356, 2009

4 Chu KH, "Rapid DNA barcoding analysis of large datasets using the composition vector method" 10 : 14-S8, 2009

5 Tamura K, "MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0" 24 : 1596-1599, 2007

6 Elias M, "Limited performance of DNA barcoding in a diverse community of tropical butterflies" 274 : 2881-2889, 2007

7 Goole maps, "Goole maps"

8 Folmer O, "DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates" 3 : 294-299, 1994

9 Ward RD, "DNA barcoding Australia's fish species" 360 : 1847-1857, 2005

10 Kress WJ, "DNA barcodes: genes, genomics, and bioinformatics" 105 : 2761-2762, 2008

11 Hajibabaei M, "DNA barcodes distinguish species of tropical Lepidoptera" 103 : 968-971, 2006

12 Hebert PD, "Biological identifications through DNA barcodes" 270 : 313-321, 2003

13 McGinnis S, "BLAST: at the core of a powerful and diverse set of sequence analysis tools" 32 : 20-W25, 2004

14 Kimura M, "A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences" 16 : 111-120, 1980