식물생장조절제 및 광도가 국화 경정배양에 미치는 영향

국문 초록 (Abstract)

생장조절물질과 광도가 국화 경정배양에 미치는 영향 을 조사하였다. 다신초(multiple shoots) 형성은 국화 경 정을 MS배지에 kinetin 2.0mg·L-1와 NAA 0.2mg·L-1 첨가된 배지에 배양하였을 때 촉진되었으며, 형성된 다 신초는 MS배지에 kinetin 2.0mg·L-1와 NAA 0.02 mg·L-1 첨가된 배지에 배양하였을 때 생육이 촉진되었 다. 한편, 국화 경정을 NAA 0.2mg·L-1와 BA 1.0mg·L- 1가 첨가된 배지에 배양하였을 경우에는 절편체당 신초 형성 수가 6.9개로 가장 많았으나 형성된 신초의 대부분 이 유리질화 되었다. 기내에서 국화묘의 생장은 광도 100 μmol·m-2·s-1 PPFD에서 신초의 길이와 엽록소 함량이 가장 높았다.

다국어 초록 (Multilingual Abstract)

Effect of plant growth regulators and light intensity on the shoot tip culture for virus free stock was investigated in chrysanthemum (Dendranthema grandiflorum) cv. Beckson. Multiple shoots from shoot tip cultures were induced on MS medium supplemented with 0.2 mg·L-1 NAA and 2.0 mg·L-1 kinetin. Subsequent growth of multiple shoots clump was much better on MS medium supplemented with 0.02 mg·L-1 NAA and 2.0 mg·L-1 kinetin. However, shoot tip cultures showed the greatest number of shoots (6.9 shoots) on MS medium with 0.2 mg·L-1 NAA and 1.0 mg·L-1 BA, but these shoots resulted to vitrification. When shoots were cultured under 100 μm m-2 s-1 photosynthetic photon flux density (PPFD), shoot length and chlorophyll content were more improved as compared to the others PPFD treatments.

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