人蔘Saponin이 Prolactin分泌에 미치는 效果

다국어 초록 (Multilingual Abstract)

The present study is involved with the prolactin secretion from anterior pituitary gland by ginseng saponin since it was handled down by tradition that Ginseng might influence on the milk secretion when it was given to nursing mother. To investigate the effect of saponin on the prolactin production or release from the anterior pituitary gland, cell culture study and whole animal studys were carried out.
For the cell culture study, enzymatically dispersed anterior pituitary cells which were obtained by dispersion the rat anterior pituitary gland in HEPES buffers containing trypsin were used. Ginseng saponin was added to the culture media and the amount of prolactin produced in the cell culture media were determined by radioimmunoassay (RIA) technique. Dose-dependent increases of prolactin with ginseng saponin were observed, whereas, no change were observed without ginseng treatment.
For the whole animal study, normal and castrated rats which previously cannulated into the heart via the right juglar vein were used. The prolactin concentration in plasma were determined by using the technique of RIA.
In normal rats, prolactin concentration in plasma were elevated dramatically after 1hour with ginseng saponin administration, whereas, instantaneous increases were observed in castrated rat. For prolactin assay by RIA, NIAMDD Rat prolactin kit NIAMDD Rat prolactin RP-1 were used as standard. The results indicate that ginseng saponin increased the release of prolactin from the anterior pituitary gland and production of prolaction from the cell in rats.

목차 (Table of Contents)

• Abstract
• I. 緖論
• II. 實咸材科 및 方法
• 1. 人蔘Saponin의 製造
• 2. 腦下垂體前葉細胞培養에 依한 Prolactin의 生成
• 3. 自鼠 血漿內 Prolaction의 濃度
• 4. 放射線免疫分析法(R.I.A)
• III. 結論
• IV. 實驗結果 및 考察
• 參考文獻