전처리 방법이 무청의 표면 미생물 변화에 미치는 영향 = Effects of Pre-treatment Method on the Surface Microbes of Radish (Raphanus sativus L.) Leaves

다국어 초록 (Multilingual Abstract)

It was investigated the effects of pre treatment method on the microbes on the surface of radish (Rapharnus sativus L.) leaves. Independent variables put in water washing (X1), microwave treatment (X2) and steam treatment (X3) using central composite design and response surface analysis. It was not detected in the pathogenic microbes, Samonella spp., Camphylobacter spp., Vibrio spp., Shigella spp., Staphyloccocus spp., on the surface of collected radish leaves without pre treatment. But general microbes showed 3.90×105∼1.20×107 CFU/g of total microbes, 1.10×102∼2.00×105 CFU/g of E. coli, 2.40×103∼3.55×106 CFU/g of yeast/mold on the surface of various radish leaves and lactic acid bacteria was detected or not according to collected samples. The best method of pre treatment was steam treatment on the microbe reduction effect of samples surface. Also, the multiplex regression coefficients analysis was calculated three independent variables (X1, X2, X3) and dependent variables (total microbes, lactic acid bacteria and yeast/molds). It showed high correlation R2, 0.89, 0.87, 0.85, respectively. For effective reduction of surface microbes, the best method was water washing with microwave or steam treatment at the same time.

국문 초록 (Abstract)

품종별 무청의 표면 미생물 분포와 중심합성계획과 반응표면분석법을 이용하여 물세척, 마이크로웨이브처리 및 스팀처리를 독립변수로 두고, 이들 전처리 조건에 따른 표면 미생물의 변화를 조사하였다. 그 결과 무청 품종에 관계없이 병원성 미생물인 Salmomella spp., Camphylobacter spp., Vibrio spp., Shigella spp., Staphylococcus spp., Enterococcus spp. 및 Pediococcus spp.는 전혀 검출되지 않았다. 그러나 총균수의 경우는 품종에 따라 약간의 차이는 있었는데, 3.90×105∼1.20×107 CFU/g의 범위를 보였고, 대장균 군은 1.11×102∼2.00×105 CFU/g, 효모/곰팡이는 2.40×103 ∼3.55×106 CFU/g을 보인 반면 젖산균은 검출되지 않은 시료도 있었다. 또 전처리 방법에 따른 무청의 잔존 미생물의 감균 효과가 가장 좋은 전처리 방법은 스팀처리 방법이었다. 원료 무청의 각각의 전처리 방법을 독립변수(independent variables)로 하고, 총균수, 젖산균수 및 효모/곰팡이균수를 종속변수(dependent variables)로 하여 회귀 분석한 결과 상관관계를 나타내는 R2 값이 각각 0.89, 0.87, 0.85로 상관성이 높았으며, 물세척 횟수에 의한 감균 효과는 제한적이므로 마이크로웨이브처리나 스팀처리를 병행하여야 효과적이었다.

참고문헌 (Reference)

1 "Survival and growth of Enterobacter sakazaki on fresh-cut fruits and vegetables and in unpasteurized juices as affected by storage temperature" 68 : 2541-2552, 2005

2 "Quality changes and pasteurization effects of citrus fruit juice by high voltage pulsed electric fields (PEF) treatment" 35 : 635-641, 2003

3 "Pathogenic microorganisms associated with fresh produce" 59 : 204-216, 1996

4 "Microbial assessment of wild cabbage and its control" 36 : 162-167, 2004

5 "Library of congress Catalog card No" 57-5903 378, 1957

6 "Isolation of Listeria monocytogenes from vegetation" 95 : 300-320, 1986

7 "Intra-cellular pH survival of Listeria monocytogenes Scott A and effect in trypic soy broth containing acetic" 15-19, 1991

8 "Inhibition of Salmonella by sodium nitrite and potassium sorbate in frankfurters" 47 : 1615-1617, 1982

9 "Incidence of Listeria monocytogenes in nature" 6 : 29-32, 1975

10 "Inactivation of Lactobacillus plantarum by pulsed-microwave irradiation" 62 : 163-166, 1997

11 "Hemorrhagic colitis associated with a rate Escherichia coli serotype" 681-685, 1983

12 "H7 ranks as the fourth most costly foodborne disease" 51-59, 1993

13 "Food pasteurization using high-intensity pulsed electric fields" 49 : 55-60, 1995

14 "Food Composition Table" National Rural Living Science Institute, 100-102, 1996

15 "Enhanced inhibition of Listeria monocytogenes by glycerol monolaurate with organic acids" 59 : 1258-1261, 1994

16 "Effects of different disinfection treatments on the natural microbiota of lettuce" 66 : 1697-1700, 2003

17 "Detection of Listeria spp in faces from animals and in raw foods of animal origin" 229-242, 1989

18 "Design and Analysis of Sensory Optimization" Food & Nutrition Press Inc 1993

19 "Combination effects of benzoate sorbate and pH for control of Escherichia coli O157 H7" 12 : 200-207, 1997

20 "Antimicrobial effects of organic acids and ethanol on several foodborne microorganisms" 31 : 1315-1323, 1999

21 "A note on the microbiology of retail packs of salad vegetables" 63 : 409-415, 1987