어성초의 아임계수 추출물의 항산화 활성 및 Acetylcholinesterase 저해 활성

국문 초록 (Abstract)

어성초(H. cordata Thunb) 건조 분말(0.05 g)을10 mL의 증류수와 함께 스테인리스 용기에 넣고 밀봉한 후 여러 온도(50, 100, 200, 300℃)에서 10, 30, 60분간 추출하였다. 추출온도와 시간은 어성초 추출물의 중요성분, 항산화 활성, acetylcholinesterase 저해 활성에 크게 영향을 미쳤다. 300℃에서 30분간 처리한 어성초 추출물에서 가장 높은 페놀함량(33.34±0.96 μg gallic acid equivalents/mL), quercetin 함량(15.75±0.20 μg/mL), DPPH 라디칼 소거능(44.07±2.65%), acetylcholinesterase 저해 활성(36.53±1.14%)이 나타났다. 플라보노이드 함량은 100℃ 10분 추출물에서 2.55 μg quercetin equivalents/mL로 가장 높게 측정되었다. 100℃ 이상의 고온에서 밀폐된 증류수는 높은 압력에서 아임계수 형태로 존재하는데 이러한 아임계수는 고품질의 어성초 추출물을 제조하는데 매우 유용하였다.

다국어 초록 (Multilingual Abstract)

Houttuynia cordata Thunb powder (0.05 g) was placed in a stainless vessel containing 10 mL of water, and subcritical water (SCW) extraction was carried out at various temperatures (50, 100, 200, and 300℃) for 10, 30, and 60 min. SCW treatment significantly affected physiologically important compounds, antioxidant activity, and acetylcholinesterase inhibitory activity of H. cordata Thunb extracts. H. cordata Thunb extracted at 300℃ for 30 min had the highest quercetin content, DPPH radical scavenging activity, total phenolic content, and acetylcholinesterase inhibitory activity, whereas the extract at 100℃ for 10 min possessed the highest flavonoid content. The highest ABTS radical scavenging activity was found in H. cordata Thunb extracted at 200 and 300℃ for 60 min. These results indicate that SCW extraction might be a useful processing method for obtaining valuable materials from H. cordata Thunb.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• 감사의 글
• 문헌

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