정향 꽃봉오리 에탄올 추출물의 Porphyromonas gingivalis 내독소를 처리한 사람 치은섬유모세포에 대한 항염 효과 = Anti-inflammatory effects of ethanol extracts of the flower buds of Syzygium aromaticum L. on human gingival fibroblast cells treated with Porphyromonas gingivalislipopolysaccharide

다국어 초록 (Multilingual Abstract)

This study was conducted to evaluate the cytotoxic and anti-inflammatory effects of the ethanol extract of Syzygium aromaticumL. (clove) buds. The cytotoxicity test was performed by cell counting method using hTERT-hNOF cells, a human immortalizedgingival fibroblast cell line. To test the anti-inflammatory effects, the hTERT-hNOF cells were treated with lipopolysaccharide(LPS) extracted from Porphyromonas gingivalis KCOM 2804 (PgLPS) and ethanol extract of clove buds. The expression levelsof PGE2, IL-6, and IL-8 were measured by enzyme-linked immunosorbent assay. The cytotoxicity test data showed a cell viabilityof ≧ 82% in hTERT-hNOF cells treated with 10 to 80 µg/mL of the ethanol extract of clove buds. The anti-inflammatory testdata showed that the expression of PGE2 by PgLPS treatment was reduced to the level of the negative control group by treatmentwith 10 µg/mL or more of the ethanol extract of clove buds. In group treated with PgLPS and 40 µg/mL of clove bud ethanolextract, the expression levels of IL-6 and IL-8 in were inhibited by 75% and 77%, respectively (p<0.05), compared to the positivecontrol (PgLPS treatment) group. These results suggest that the ethanol extract of clove buds can be used in developing oralhygine products for preventing periodontal disease.