Optimization of lipid extraction from marine green macro-algae as biofuel resources

다국어 초록 (Multilingual Abstract)

The extraction of lipid from Enteromorpha intestinalis was investigated. Among 13 types of organic solvent, the chloroform/methanol (2 : 1, v/v) system obtained the highest content. Considering the lipid extraction procedure and extracted amount, the optimal solid/liquid ratio was 1 : 50 (w/v). With increasing extraction temperature, the content was enhanced until 45 oC, after which it increased only slightly. Correspondingly, with increasing extraction time, the content linearly increased until 2.5 hr, after which it held steady. From a fatty acid compositional analysis of the lipid extracted from E. intestinalis, the content of palmitic acid and stearic acid was 39.85±1.19% and 14.61±0.80%, respectively. The unsaturated fatty acids (C18:1, C18:2 and C18:3) concentrations, meanwhile, were 0.3-3.2%.

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