레티노인산 결핍에 의한 사람 중이점막 상피세포의 각화편평상피세포로의 분화 = Retinoic Acid Depletion Induces Keratinizing Squamous Differentiation in Human Middle Ear Epithelial Cell Culture

다국어 초록 (Multilingual Abstract)

Background and Objectives：The pathogenesis of cholesteatoma behind an intact tympanic membrane remains controversial. Squamous metaplasia of the midle ear mucosa is thought to be a possible mechanism in such cases. However, to date, no definitive experimental results have proved the asociation. This study was undertaken to investigate whether normal human midle ear epithelial (NHME ) cells undergo keratinizing squamous differentiation in retinoic acid (RA)-deficient culture. Materials and Method：determined the expresions of the mucin gene and cornifin-α mRNAs as indicators of mucous and squamous differentiation, respectively. Results：Histomorphologicaly, the NHMEE cells diferentiated into a keratinizing squamous epithelium in RA-deficient culture. In addition, the expressions of mucin gene 5AC (MUC5AC) and MUC8 mRNA were suppressed, and the expression of cornifin-α mRNA increased progresively as a function of differentiation in RA-deficient culture. Conclusion：Our study shows that RA depletion induces keratinizing squamous diferentiation in NHMEE cell culture. (Korean J Otolaryngol 2003 ;46 :464-8)