Genetic variation of COI gene of the Korean medicinal centipede Scolopendra mutilans Koch, 1878 (Scolopendromorpha: Scolopendridae)

다국어 초록 (Multilingual Abstract)

In Korea, the centipede called “Wang‐ji‐ne” or “O‐gong” is used as an important medicinal resource. This centipede has been known as Scolopendra subspinipes mutilans Koch 1878. Recent studies have assessed its taxonomic treatment in several geographical populations from China, Japan and Taiwan, but not Korea. We therefore attempted to assess exact species status for the Korean population of this subspecies using both morphological and DNA barcode methods. The result inferred from DNA barcoding showed that the Korean population is S. mutilans explicitly separated from S. subspinipes. Within S. mutilans, the Korean population is morphologically identical and genetically closer to the Chinese population rather than island populations of Japan and Taiwan. Particularly, the mainland populations from Korea and China share six haplotypes from 17 despite being far apart geographically.

참고문헌 (Reference)

1 Schileyko AA, "The scolopendromorph centipedes (Chilopoda) of Vietnam, with contributions to the faunas of Cambodia and Laos. Part 3" 16 : 71-95, 2007

2 Chao JL, "The scolopendromorph centipedes (Chilopoda) of Taiwan" 44 : 1-11, 2003

3 SiriwutW, "The centipede genus Scolopendra in Mainland Southeast Asia: Moleculdar phylogenetics, geometric morphometrics and external morphology as tools for species delimitation" 10 : e0135355-, 2015

4 Saitou N, "The Neighbor-joining method: a new method for reconstructing phylogenetic trees" 4 : 406-425, 1987

5 Paik KY, "The Myriapods fauna of Quelpart Island, Korea" 7 : 33-42, 1961

6 Kang S, "Taxonomy and identification of the genus Scolopendra in China using integrated methods of external morphology and molecular phylogenetics" 7 : 16032-, 2017

7 Lee HG, "Taxonomical studies on class Chilopoda from Korea" Wonkwang University 1989

8 Park H, "Survey report of food and medicinal insects in Japanese occupation of Korea" National Institute of Agricultural Science, RDA 2017

9 Schileyko AA, "Scolopendromorph centipedes of Vietnam (Chilopoda Scolopendromorpha). Part 2" 4 : 73-87, 1995

10 Kraepelin K, "Revision der Scolopendriden" 29 : 1-276, 1903

11 Kronmüller C, "Review of the subspecies of Scolopendra subspinipes Leach, 1815 with the new description of the South Chinese member of the genus Scolopendra Linnaeus, 1758 named Scolopendra hainanum spec. nov. (Myriapoda, Chilopoda, Scolopendridae)" 35 : 19-27, 2012

12 Vahtera V, "Phylogentics of Scolopendromorph centipedes: can denser taxon sampling improve an artificial classification?" 27 : 578-602, 2013

13 Hong ND, "Pharmacological studies of Scolopendra subspinipes mutilans L. Koch" 7 : 99-109, 1976

14 Paik KY, "On the Myriapods of Mt. Jiri" 3 : 5-13, 1960

15 Oh C, "Medicinal animal science" Uisungdang Seoul 2002

16 Tamura K, "MEGA5:Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods" 28 : 2731-2739, 2011

17 Koch L, "Japanesische archniden und Myriapoden" 27 : 735-795, 1878

18 International Commission on Zoological Nomenclature, "International code of Zoological nomenclature" International Trust for Zoological Nomenclature, c/o The National History Museum 1999

19 Letunic I, "Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees" 44 : W242-W245, 2016

20 Pemberton RW, "Insects and other arthropods used as drugs in Korean traditional medicine" 65 : 207-216, 1999

21 Murienne J, "Including secondary structure, fossils and molecular dating in the centipede tree of life" 57 : 301-313, 2010

22 Vahtera V, "Evolution of blindness in scolopendromorph centipedes (Chilopoda: Scolopendromorpha): insight from an expanded sampling of molecular data" 28 : 4-20, 2012

23 Folmer O, "DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates" 3 : 294-299, 1994

24 Kong Y, "Cytotoxic and anticoagulant peptide from Scolopendra subspinipes mutilans venom" 7 : 2238-2245, 2013

25 Takakuwa Y, "Class Chilopoda, Epimorpha, Scolopendromorpha" 9 : 1-81, 1940

26 Takakuwa Y, "Chilopods and diplopods known from Korea" 5 : 164-185, 1940

27 Hakim MA, "Centipede venoms and their components: resources for potential therapeutic applications" 7 : 4832-4851, 2015

28 Hebert PDN, "Biological identifications through DNA barcodes" 270 : 313-321, 2003

29 이준하, "Antimicrobial Activity of the Scolopendrasin V Peptide Identified from the Centipede Scolopendra subspinipes mutilans" 한국미생물·생명공학회 27 (27): 43-48, 2017

30 Siriwut W, "A taxonomic review of the centipede genus Scolopendra Linnaeus, 1758 (Scolopendromorpha, Scolopendridae) in mainland Southeast Asia, with description of a new species from Laos" 590 : 1-124, 2016

31 Leach WE, "A tabular view of the external characters of four classes of animals, which Linné arranged under Insecta; with the distribution of the genera composing three of these classes into orders and descriptions of several new genera" 11 : 306-400, 1816

32 Kimura M, "A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences" 16 : 111-120, 1980

33 Paik KY, "A list of Chilopoda, Symphila and Diplopoda from Korea" 2 : 351-369, 1958

34 Lewis JGE, "A key and annotated list of the Scolopendra species of the Old World with a reappraisal of Arthrorhabdus (Chilopoda: Scolopendromorpha: Scolopendridae)" 3 : 83-122, 2010

35 장태호, "(The)Classification of Korean centipedes(class chilopoda)" 江陵大學校 大學院 1996