Amino acid digestibility in diets containing copra meal with β-mannanase fed to growing pigs

다국어 초록 (Multilingual Abstract)

Objective: The objective of this study was to estimate standardized ileal digestibility (SID) of amino acids (AA) in growing pigs fed diets containing increasing levels of copra meal (CM) with β-mannanase supplementation.Methods: Twenty barrows (initial body weight: 34.43±0.11 kg) surgically fitted with T-cannulas at the distal ileum were individually housed in metabolism crates. Pigs were allotted to 5 dietary treatments in a completely randomized design with 4 replicates per treatment. The dietary treatments were: i) NC, negative control, corn-soybean meal (SBM) based diet, ii) PC, positive control, basal diet + 0.10% β-mannanase supplementation (800 IU/kg), iii) CM6, PC diet with 6% CM supplementation, iv) CM12, PC diet with 12% CM supplementation, and v) CM18, PC diet with 18% CM supplementation. A nitrogen-free diet was used to estimate basal endogenous losses of AA for SID calculation. All experimental diets contained 0.5% chromic oxide as an indigestible marker. Each period consisted of a 4-d diet adaptation period and a 3-d ileal digesta collection period.Results: There were no differences in apparent ileal digestibility (AID) and SID of all AA between the NC and PC treatments except that the PC treatment had lower AID and SID of glycine than the NC treatment (p<0.05). There were linear decreases in AID and SID of lysine (p<0.05) and aspartic acid (p = 0.06; tendency) with increasing levels of CM in the diets with β-mannanase.Conclusion: The β-mannanase supplementation had no effect on AA digestibility in pigs fed the corn-SBM based diet but increasing levels of CM reduced SID of lysine and aspartic acid.

다국어 초록 (Multilingual Abstract)

Objective: The objective of this study was to estimate standardized ileal digestibility (SID) of amino acids (AA) in growing pigs fed diets containing increasing levels of copra meal (CM) with β-mannanase supplementation.
Methods: Twenty barrows (initial body weight: 34.43±0.11 kg) surgically fitted with T-cannulas at the distal ileum were individually housed in metabolism crates. Pigs were allotted to 5 dietary treatments in a completely randomized design with 4 replicates per treatment. The dietary treatments were: i) NC, negative control, corn-soybean meal (SBM) based diet, ii) PC, positive control, basal diet + 0.10% β-mannanase supplementation (800 IU/kg), iii) CM6, PC diet with 6% CM supplementation, iv) CM12, PC diet with 12% CM supplementation, and v) CM18, PC diet with 18% CM supplementation. A nitrogen-free diet was used to estimate basal endogenous losses of AA for SID calculation. All experimental diets contained 0.5% chromic oxide as an indigestible marker. Each period consisted of a 4-d diet adaptation period and a 3-d ileal digesta collection period.
Results: There were no differences in apparent ileal digestibility (AID) and SID of all AA between the NC and PC treatments except that the PC treatment had lower AID and SID of glycine than the NC treatment (p<0.05). There were linear decreases in AID and SID of lysine (p<0.05) and aspartic acid (p = 0.06; tendency) with increasing levels of CM in the diets with β-mannanase.
Conclusion: The β-mannanase supplementation had no effect on AA digestibility in pigs fed the corn-SBM based diet but increasing levels of CM reduced SID of lysine and aspartic acid.

참고문헌 (Reference)

1 H.J.Kim, "Various levels of copra meal supplementation with β-Mannanase on growth performance, blood profile, nutrient digestibility, pork quality and economical analysis in growing-finishing pigs" 한국축산학회 59 (59): 1-10, 2017

2 Stein HH, "Technical note : A technique for inserting a T-cannula into the distal ileum of pregnant sows" 76 : 1433-1436, 1998

3 McCleary BV, "Synthesis of β-D-mannosidase and exo-β-Dmannanase" 160 : 515-518, 1988

4 Jang YD, "Short Communication : Energy values and apparent total tract digestibility coefficients of copra meal and palm kernel meal fed to growing pigs" 93 : 517-521, 2013

5 "Official methods of analysis of AOAC International. 18th ed"

6 Stein HH, "Nutritional value of high fiber co-products from the copra, palm kernel, and rice industries in diets fed to pigs" 6 : 56-, 2015

7 Committee on Nutrient Requirements of Swine, National Research Council, "Nutrient requirements of swine" National Academy Press 2012

8 Committee on Nutrient Requirements of Swine, National Research Council, "Nutrient requirements of swine" National Academy Press 1998

9 Jondreville C, "Ileal digestibility of amino acids in feedstuffs for pigs" Eurolysine/ITFC publication 53-, 1995

10 Moughan PJ, "Endogenous amino acid flow in the stomach and small intestine of the young growing pig" 60 : 437-442, 1992

11 Jang JC, "Effects of increasing levels of palm kernel meal containing β-mannanase to growingfinishing pig diets on growth performance, nutrient digestibility, and pork quality" 238 : 104041-, 2020

12 Jang JC, "Effects of copra meal inclusion level in growing-finishing pig diets containing β-mannanase on growth performance, apparent total tract digestibility, blood urea nitrogen concentrations and pork quality" 10 : 1840-, 2020

13 Kim BG, "Effect of partial replacement of soybean meal with palm kernel meal and copra meal on growth performance, nutrient digestibility and carcass characteristics of finishing pigs" 14 : 821-830, 2001

14 Odetallah HN, "Effect of mannan-endo-1, 4-β-mannosidase on the growth performance of turkeys fed diets containing 44 and 48%crude protein soybean meal" 81 : 1322-1331, 2002

15 Fan MZ, "Determination of apparent ileal amino acid digestibility in barley and canola meal for pigs with the direct, difference, and regression methods" 73 : 2364-2374, 1995

16 Jeon SM, "Comparative standardized ileal amino acid digestibility and metabolizable energy contents of main feed ingredients for growing pigs when adding dietary β-mannanase" 5 : 359-365, 2019

17 Lee SA, "Classification of copra meal and copra expellers based on ether extract concentration and prediction of energy concentrations in copra byproducts" 27 : 34-39, 2017

18 Saittagaroon S, "Characterisation of polysaccharides of copra meal" 34 : 855-860, 1983

19 A.O. Adebiyi, "Apparent or Standardized Ileal Digestibility of Amino Acids of Diets Containing Different Protein Feedstuffs Fed at Two Crude Protein Levels for Growing Pigs" 아세아·태평양축산학회 28 (28): 1327-1334, 2015

20 Fenton TW, "An improved procedure for the determination of chromic oxide in feed and feces" 59 : 631-634, 1979

21 손아름, "Amino acid digestibility in plant protein sources fed to growing pigs" 아세아·태평양축산학회 32 (32): 1745-1752, 2019

22 Son AR, "Amino acid digestibility in copra expellers and palm kernel expellers by growing pigs" 187 : 91-97, 2014

23 Sulabo RC, "Amino acid digestibility and concentration of digestible and metabolizable energy in copra meal, palm kernel expellers, and palm kernel meal fed to growing pigs" 91 : 1391-1399, 2013

24 Jorgensen H, "Amino acid availabilities in soybean meal, sunflower meal, fish meal and meat and bone meal fed to growing pigs" 58 : 926-934, 1984