Comparative Analysis of Tissue and Cell Cycle on the Far Eastern Catfish, Silurus asotus between Diploid and Triploid

다국어 초록 (Multilingual Abstract)

The influence of triploidization on histological characteristics of retina, trunk kidney, liver and midgut tissue, and cell cycle of tail fin and gill tissue in far eastern catfish, Silurus asotus were analyzed. In the infertile triploid fish, the nu-cleus and/or cell size of secondary proximal tubule cells of trunk kidney, hepatocyte and midgut epithelium are much larger than those of the corresponding cells in the diploid fish (P<0.05). However, triploid tissue showed fewer number of outer nu-clear layer in retina and nuclei in secondary proximal tubule of trunk kidney than those for diploid tissue. The mean percent-ages of the Gl-, the S- and the G2+M-phase fractions were 92.5%, 3.2% and 4.3% in tail fin tissue of diploid, and 93.4%, 2.6% and 4.0% in those of triploid, respectively. There were no significant differences in the percentages of each cell cycle fraction between diploid and triploid. The mean percentages of each phase fractions were 75.1%, 11.1% and 13.8% in gill tissue of diploid and 85.2%, 8.9% and 5.9% in those of triploid, respectively. The differences of cell cycle between tail fin tissue and gill tissue were statistically significant in diploid and triploid (P<0.05). Also, the differences between diploid and triploid were statistically significant in tail fin tissue and gill tissue (P<0.05). Cyclin D1 and cyclin E expressions were not significant-ly difference between gill tissue and tail fin tissue, and protein expressions of induced triploid were higher than those of di-ploid. Results from this study suggest that some characteristics in the triploid exhibiting larger cell and nucleus size with fewer number of cell than diploid can be used as an indicator in the identification of triploidization and ploidy level in far eastern catfish.

참고문헌 (Reference)

1 박인석, "한국의 메기과(Siluridae) 어류의 계측형질 특징" 한국어류학회 16 (16): 223-228, 2004

2 Estevam J, "Validation of a flow cytometry based G2M delay cell cycle assay for use in evaluating the pharma-codynamic response to Aurora A inhibition" 363 : 135-142, 2011

3 Kim DS, "Triploidy produc-tion of nile tilapia(Oreochromis niloticus)" 3 : 135-144, 1990

4 Thorgaard GH, "Triploid rainbow trout identified by flow cytometry" 29 : 305-309, 1982

5 Maxime V, "The physiology of triploid fish : current knowledge and comparisons with diploid fish" 9 : 67-78, 2008

6 Benfey TJ, "The physiology and behavior of triploid fishes" 7 : 39-67, 1999

7 Don J, "The induction of triploidy in Oreochromis aureus by heat shock" 72 : 186-192, 1986

8 Tiwary BK, "The biology of triploid fish" 14 : 391-402, 2004

9 Takashi H, "Sensory organs" Kodansha Ltd 42-53, 1982

10 Allen SK, "Reproductive sterility in polyploidy brook trout(Salvelinus fontinalis)" 107 : 473-478, 1978

11 Cassani JR, "Problems associated with tetraploid induction and sur-vival in grass carp(Ctenopharyngodon idella)" 88 : 273-284, 1990

12 Thorgaard GH, "Ploidy manipulation and perfor-mance" 57 : 57-64, 1986

13 Dean PN, "Mathematical analysis of DNA distributions derived from flow microfluorometry" 60 : 523-527, 1974

14 Park I-S, "Karyotypes and cellular DNA contents of two species in the genus Lateolabrax from Korea" 65 : 488-489, 1999

15 Kim DS, "Induction of triploidy in mud loach(Misgurnus mizolepis)and its effect on gonad development and growth" 120 : 263-270, 1994

16 Park I-S, "Induction of triploid red seabream(Pagrus major)" 6 : 71-78, 1994

17 Park I-S, "Induction of triploid cherry salmon(Oncorhynchus masou)" 7 : 176-192, 1994

18 Ueno K, "Induction of triploid carp and their haema-tological characteristics" 59 : 585-591, 1984

19 Choi GC, "Induced breeding and indoor culture of the catfish(Silurus aso-tus)(Teleostomi : Siluridae)" 5 : 117-126, 1992

20 양원석, "Identification of Skeletal Deformities in Far Eastern Catfish, Silurus asotus under Indoor Aquaculture Condition" 한국발생생물학회 19 (19): 153-161, 2015

21 Yamashita Y, "Histological change in the muscles of diploid and triploid ayu during growth and maturation" 59 (59): 151-154, 1993

22 Park I-S, "Haematological and physio-logical characteristics of diploid and triploid in cherry salmon(Oncorhynchus masou)" 7 : 193-201, 1994

23 설동원, "Haematological Parameters and Respiratory Function in Diploid and Triploid Far Eastern Catfish, Silurus asotus" 한국유전학회 30 (30): 205-213, 2008

24 Tave D, "Growth of triploid and diploid bighead carp(Hypophthalmichthys nobilis)" 2 : 13-25, 1993

25 Hulata G, "Genetic manipulation in aquaculture : a review of stock improvement by classical and modern technologies" 111 : 155-173, 2001

26 Vanparys C, "Flow cytometric cell cycle analysis allows for rapid screening of estro-genicity in MCF-7 breast cancer cells" 20 : 1238-1248, 2006

27 Wolters WR, "Erythro-cyte nuclear measurements of diploid and triploid channel catfish, Ictalurus punctatus (Rafinicsque)" 20 : 253-258, 1982

28 Aliah RS, "Effects of triploidy on tissue structure of some organs in ayu" 56 : 569-575, 1990

29 Swarup H, "Effect of triploidy on the body size, ge-neral organization and cellular structure in Gasteros-teus aculeatus" 56 : 143-155, 1959

30 Park I-S, "Effect of starvation on the growth and hepatocyte nu-clear size of larval rockfish (Sebastes schlegelii) and larval spotted sea bass (Lateolabrax sp.)" 11 : 345-352, 1998

31 Komaru A, "Detection of induced triploid scallop(Chlamys nobilis)by DNA microflurometry with DAPI staining" 69 : 201-209, 1988

32 Nunez R, "DNA measurement and cell cycle analy-sis by flowcytometry" 3 : 67-, 2001

33 Kim DS, "Cytogenetic traits and gonad development of induced triploidy in far eastern catfish(Silurus asotus)" 23 : 55-62, 2001

34 Murray JD, "Comparison of traditional breeding and transgenesis in farmed fish with implications for growth enhance-ment and fitness" CAB International 209-229, 1999

35 Park I-S, "Comparison of some tissue in diploid and triploid hybrid between mud loach (Mis-gurnus mizolepis) and cyprinid loach (M. anguilli-caudatus)" 4 : 19-28, 2000

36 Xoana T, "Comparative expression analy-sis in mature gonads, liver and brain of turbot (Scoph-thalmus maximus) by cDNA-AFLPS" 492 : 250-261, 2012

37 Phillips RB, "Application of silver staining to the identification of triploid fish cells" 54 : 313-319, 1986