Morphological and molecular characterization of a new isolate of entomopathogenic nematode, Steinernema feltiae (Filipjev) (Rhabditida: Steinernematidae) from the Arasbaran forests, Iran

다국어 초록 (Multilingual Abstract)

Steinernema feltiae isolate IRAZ13 was recovered from one soil sample collected from the Arasbaran forests, Iran. The new isolate can be distinguished from other isolates by morphologic and morphometric data, and DNA sequences. The first generation male is characterized by spicule length 79 ± 5 (73-87) mm, SW ratio [(spicule length/anal body diameter)*100] 160 ± 13 (137-177), GS ratio GS [(gubernaculum/ spicule length)*100] 79 ± 8 (62-87), genital papillae arrangement and the second generation male by a mucron 10 ± 1 (9-11) mm long. For the infective juvenile, the body is 883 ± 82 (754-975) mm long, lateral fields with eight equally developed ridges, head smooth, slightly offset, large gonad primordium cells, a long hyaline tail portion (equal to half of tail length), and pore-like phasmid at 41% of the tail length to the semicircle anus are the differentiation factors. Additionally, molecular studies obtained from the 28S-D2/D3 region and ITS1 rDNA sequence analyses and phylogenetic reconstruction further support this nematode as a different isolate.

참고문헌 (Reference)

1 Vilcox D., "UTHSCSA Image Tool for windows version 3. 0" The University of Texas Health Science Center in San Antonio 2002

2 Thompson JD., "The CLUSTAL_X Windows interface : flexible strategies for multiple sequence alignment aided by quality analysis tools" 25 : 4876-4882, 1997

3 Woodring JL., "Steinernematid and Heterorhabditid nematodes : a handbook of biology and techniques. Southern cooperative series bulletin 331" Arkansas Agricultural Experiment Station 1988

4 Phan KL., "Steinernema loci sp. n. and Steinernema thanhi sp. n. (Rhabditida : Steinernematidae)from Vietnam" 3 : 503-514, 2001

5 Nikdel M., "Steinernema arasbaranense n. sp. (nematoda : steinernematidae), a new entomopathogenic nematode from Arasbaran forests, Iran" 39 : 17-28, 2011

6 Nguyen KB., "Scanning electron microscope studies of Steinernema glaseri(Nematoda : Steinernematidae)" 41 : 183-190, 1995

7 Tabassum KA., "Redescription of Steinernema feltiae Filipjev, 1934(Nematoda : Steinernematidae)from Pakistan" 22 : 1-8, 2004

8 Tanha maafi Z., "Record of two steinernematid species from Iran" 482-, 2006

9 Spiridonov SE., "Phylogenetic relationships within the genus Steinernema(Nematoda : Rhabditida)as inferred from analyses of sequences of the ITS1-5. 8S-ITS2 region of rDNA and morphological features" 6 : 547-566, 2004

10 Nadler SA., "Phylogenetic relationships of Steinernema Travassos, 1927(Nematoda : Cephalobina : Steinernematidae)based on nuclear, mitochondrial and morphological data" 63 : 161-181, 2006

11 Nguyen KB., "Neosteinernema longicurvicauda n. gen., n. sp. (Rhabditida : Steinernematidae), a parasite of the termite Reticulitermes flavipes(Koller)" 26 : 162-174, 1994

12 Posada D., "Modeltest : testing the model of DNA substitution" 14 : 817-818, 1998

13 Larget B., "Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees" 16 : 750-759, 1999

14 Huelsenbeck JP., "MR BAYES : Bayesian inference of phylogenetic tress" 17 : 1754-1755, 2001

15 Lawrence AL., "Entomopathogenic nematodes for control of insect pests above and below ground with comments on commercial production" 44 : 218-225, 2012

16 Nguyen KB., "Entomopathogenic nematodes : systematics, phylogeny and bacterial symbionts" American Phytopathological Society Press 2007

17 Nikdel M., "Diversity of entomopathogenic nematodes(Nematoda : Steinernematidae, Heterorhabditidae)from Arasbaran forests and rangelands in the north-west of Iran" 12 : 767-773, 2010

18 Hominick WM., "Biogeography. In : Gaugler R, editor. Entomopathogenic nematology" CABI Publishing 2002

19 Eivazian Kary N., "A survey of entomopathogenic nematodes of the families Steinernematidae and Heterorhabditidae(Nematoda : Rhabditida)in the north-west of Iran" 11 : 107-116, 2009