The influence of Lactobacillus rhamnosus GG on the binding ability of Streptococcus mutans = Lactobacillus rhamnosus GG가 Streptococcus mutans의 부착능에 미치는 영향

다국어 초록 (Multilingual Abstract)

Probiotics has currently attracted for means of preventive treatment measurement instead of using non-specific and broad spectrum antimicrobials. In previous studies, two main probiotics species, Lactobacillus and Bifidobateria, showed the reduction of DMFS and S. mutans counts. However, the timing of introducing probiotic species to oral cavity is not clear. The aim of this study is to evaluate the changes of binding ability of S. mutans in various concentrations and inoculation time of L. rhamnosus GG. Adding the following concentration of L. rhamnosus GG, 1×106 CFU, 1×107 CFU and 1×108 CFU, to S. mutans medium demonstrates significant reduction of S. mutans counts. Additionally, more reduction was observed when L. rhamnosus were inoculated prior to S. mutans or simultaneously inoculated compared to when S. mutans were inoculated prior to L. rhamnosus after 3 hours of incubation. Based on this research, the timing of introducing probiotics should be considered when probiotics are utilized as a preventive treatment measurement.

국문 초록 (Abstract)

최근 들어, 수복 치료 등을 이용한 질병의 직접적인 치료보다는 예방 치료에 대한 중요성이 강조되면서, 그 동안 사용되었 던화학적인 약물을 통한 예방 치료 이외에도 유산균등을 이용한 probiotics에 대한관심이 높아지고 있다. 여러 논문에서 Lactobacillus와 Bifidobateria들이 DMFS와 S. mutans를 감소한다고 보고하고 있다. 하지만, 이러한 미생물들의 접종 시 기와 접종 농도에 대한 언급은 부족하다. 따라서 본 실험에서는 probiotics의 대표적인 균인, L. rhamnosus GG를 이용하여 예방 효과를 보기 위한 적정 농도를 재정립하고, 접종시기를 고찰하였다. S. mutans 배지에 1×106 CFU이상 농도의 L. rhamnosus를 접종하였을 때 S. mutans count가 현저하게 감소하는 것을 볼 수 있었다. 접종 순서는 L. rhamnosus와 S. mutans를 동시에 접종하거나 L. rhamnosus를 S. mutans 보다 선행하여 접종하였을 때가, S. mutans를 L. rhamnosus 보다 선행하여 접종하였을 때에 비교하여 S. mutans count를 더 감소시켰다. 이 실험의 결과로 보아 probiotics의 접종 시 기는 probiotics을 임상에 적용할 때 반드시 고려되어야 하겠다.

참고문헌 (Reference)

1 신혜성, "유산균 발효유가 Streptococcus mutans의 생균수 및 biofilm 형성에 미치는 영향" 대한소아치과학회 36 (36): 358-366, 2009

2 이난영, "소아에서 Lactococcus lactis 1370에 의한 치태형성 억제 효과" 대한소아치과학회 28 (28): 583-592, 2001

3 McMurry LM, "Triclosan targets lipid synthesis" 394 : 531-532, 1998

4 Emilson CG, "Susceptibility of various microorganisms to chlorhexidine" 85 : 255-265, 1977

5 Caglar E, "Short-term effect of ice-cream containing bifidobacterium lactis bb-12 on the number of salivary mutans streptococci and lactobacilli" 66 : 154-158, 2008

6 Meurman JH, "Probiotics: Do they have a role in oral medicine and dentistry" 113 : 188-196, 2005

7 Alfaleh K, "Probiotics reduce the risk of necrotizing enterocolitis in preterm infants: A meta-analysis" 97 : 93-99, 2009

8 Haukioja A, "Probiotic bacteria affect the composition of salivary pellicle and streptococcal adhesion in vitro" 23 : 336-343, 2008

9 Nyvad B, "Microbial colonization of human tooth surfaces" 32 : 1-45, 1993

10 Sintes JL, "Enhanced anticaries efficacy of a 0.243% sodium fluoride/10% xylitol/silica dentifrice: 3-year clinical results" 8 : 231-235, 1995

11 Caglar E, "Effect of yogurt with bifidobacterium dn-173 010 on salivary mutans streptococci and lactobacilli in young adults" 63 : 317-320, 2005

12 Nase L, "Effect of long-term consumption of a probiotic bacterium, lactobacillus rhamnosus gg, in milk on dental caries and caries risk in children" 35 : 412-420, 2001

13 Lee YK, "Displacement of bacterial pathogens from mucus and caco-2 cell surface by lactobacilli" 52 : 925-930, 2003

14 Caglar E, "Bacteriotherapy and probiotics’role on oral health" 11 : 131-137, 2005

15 Petti S, "Antibacterial activity of yoghurt against viridans streptococci in vitro" 53 : 985-990, 2008

16 Caglar E, "A probiotic lozenge administered medical device and its effect on salivary mutans streptococci and lactobacilli" 18 : 35-39, 2008

17 Anderson MH, "A probiotic approach to caries management" 28 : 151-153, 2006

18 Clarkson JE, "A comprehensive summary of fluoride dentifrice caries clinical trials" 6 : 59-106, 1993